Rabbit anti-Mouse IgM Secondary Antibody, AP/兔抗小鼠IgM二抗，AP
货号：31333
规格：1 mL
价格：4030
产品类型：二抗
品牌：Thermo Fisher
物种：小鼠
宿主：兔
抗体亚型：IgG
荧光染料：AP(碱性磷酸酶)
| 抗体类型: | 二抗 | 同型对照： | |
| 浓度： | 0.6 mg/mL | 用法： | 1:5,000-1:50,000 (WB) |
产品详细信息Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.This antibody has been successfully used in Western blot and IP applications.Antibody Specificity: This antibody reacts with the heavy chain portion of mouse IgM. No antibody was detected against normal mouse IgG or non-immunoglobulin serum proteins. However, antibodies may cross-react with IgM from other species.Restoration and Storage: Store product at 4°C until opened. Restore with 1.0 mL distilled water (0.6 mg/mL after restoration). Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature. To judge clarity, draw product into a pasteur pippette. Store undiluted liquid at 4°C. DO NOT FREEZE. After dilution, do not use for more than one day.To extend the shelf-life of this product after reconstitution, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.Country of Origin: USA 靶标信息IgM (Immunoglobulin M) is expressed intracellularly during the early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. The isotype of a primary antibody and its application can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are also useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody
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