CRISPRcraft S.p. Cas9 Nuclease, 10 mg/mL (62 µM)（不卖））
货号：70030-1
规格：100 μg
价格：5503
产品类型：CRISPR基因编辑
品牌：Lucigen
| CRISPRcraft™ S.p. Cas9 Nuclease is a purified recombinant wild-type Streptococcus pyogenes Cas9 nuclease produced in E. coli, containing a C-terminal 6xHis tag and a C-terminal NLS to allow efficient transport to the nucleus. It is provided at high concentration (10 mg/mL, 62 µM) to enable efficient RNP delivery and compatibility with multiple delivery methods including lipid-based transfection and electroporation.CRISPRcraft S.p.Cas9 Nuclease*是在大肠杆菌中生产的纯化的重组野生型化脓性链球菌Cas9核酸酶，其含有C末端6x His标签和C末端核定位序列(NLS)，以使其高效转运至细胞核。它以高浓度(10mg/mL,62pM)提供，以实现有效的核糖核蛋白(RNP)递送和与多种递送方法的相容性，包括基于脂质的转染和电穿孔。 |
| 优点： |
| ▪Recombinant wild-typeStreptococcus pyogenesCas9 nuclease, with NLS;▪Ideal for efficient RNP-based delivery of Cas9/gRNA complexes；▪High concentration (10 mg/mL, 62 µM) compatible with multiple delivery methods； |
| 数据： |
| 图1.CRISPR RNP delivery.HEK293T cells were transfected in triplicate with RNP containing CRISPRcraft™ S.p. Cas9 Nuclease and guide components from the CRISPRcraft™ S.p. Cas9 Nuclease Control Kit targeting HPRT, using either lipofection or electroporation. Transfection conditions: TransIT-X2® Dynamic Delivery System (Mirus), 50 nM gRNA and 25 nM Cas9; Lipofectamine® RNAiMAX (ThermoFisher Scientific), equimolar Cas9 and gRNA at 25 nM; Ingenio® Electroporation Solution (Mirus), 1.5 µM gRNA and 0.75 µM Cas9, plus Alt-R® Cas9 Electroporation Enhancer (IDT). At 48 hours post-transfection, cleavage efficiency at the HPRT locus was determined using the T7E1 mismatch detection assay and is expressed as % gene modification.图2.In vivogene editing with different guide RNA sequences.HEK293T cells were independently transfected in triplicate with RNP containing CRISPRcraft™ S.p. Cas9 Nuclease and three different guides, each targeting a different loci within human HPRT. Transfections were performed using TransIT-X2® Dynamic Delivery System (Mirus), 20 nM guide and 10 nM Cas9 (2:1 guide:Cas9 molar ratio). Cleavage efficiency at each HPRT locus was determined using the T7E1 mismatch detection assay, 48 hours post-transfection. |
| 相关产品： |
| ▪CRISPRcraft™ S.p. Cas9 Nuclease, 10 mg/mL (62 µM)（货号#70020） |
技术参数 产品优点 - Recombinant wild-type Streptococcus pyogenes Cas9 nuclease, with NLS
- Ideal for efficient RNP-based delivery of Cas9/gRNA complexes
- High concentration (10 mg/mL, 62 µM) compatible with multiple delivery methods
- Extensive quality control to ensure activity and purity
产品应用 - CRISPRcraft™ S.p. Cas9 Nuclease is a purified recombinant wild-type Streptococcus pyogenes Cas9 nuclease produced in E. coli, containing a C-terminal 6xHis tag and a C-terminal NLS to allow efficient transport to the nucleus. It is provided at high concentration (10 mg/mL, 62 µM) to enable efficient RNP delivery and compatibility with multiple delivery methods including lipid-based transfection and electroporation.
- CRISPR/Cas9基因编辑试验
- 序列特异、RNA介导的dsDNA切割
- 序列富集和去除
产品类型 Gene Editing Nucleases
推荐用于 CRISPRcraft™ S.p. Cas9 Nuclease is a purified recombinant wild-type Streptococcus pyogenes Cas9 nuclease produced in E. coli, containing a C-terminal 6xHis tag and a C-terminal NLS to allow efficient transport to the nucleus. It is provided at high concentration (10 mg/mL, 62 µM) to enable efficient RNP delivery and compatibility with multiple delivery methods including lipid-based transfection and electroporation.
组成成分 CRISPRcraft™ S.p. Cas9 Nuclease contains one C-terminal nuclear localization signal (NLS) sequence and one C-terminal 6xHis tag and is provided as a 10 mg/mL (62 µM) solution or a 3.2 mg/mL (20 µM) solution.
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