CAL Fluor Red 635 Amidite
货号：BNS-5084-50,BNS-5084-100,BNS-5084-250
规格：50 µmol,100 µmol,250 mg
价格：2673.3,5205.9,11537.4
产品类型：核酸合成
品牌：LGC Biosearch Technologies
| CAL Fluor® Red 635 amidite fluoresces in the orange-red region of the visible spectrum. CAL Fluor Red 635 amidite is used for the 5' labeling of fluorogenic probes used in 5' nuclease assays, Molecular Beacons, Scorpions® Primers and other genomic assays. This amidite does not contain a DMT protecting group and can only be added to the 5' terminus of the oligo. CAL Fluor Red 635 fluorophore is an alternative for LC Red 640® dye and is quenched by BHQ®-2 dye. |
| 特性： |
| - Formula: C50H63Cl2F6N5O4P2- Molecular Weight: 1044.91- Appearance: blue solid- Absorption Maximum (Lambda Max): 616- Extinction Coefficient at Lambda max: 112000- Extinction Coefficient at 260 nm: 36500- Fluorescence Maximum: 637 |
| 产品用途： |
| Synthesis conditions: Due to the mild deprotection conditions we recommend using fast deprotecting amidites and ethyl thiol tetrazole for the activator reagent with this product.Dilution: 100 µmol/mLDeprotection conditions: For a 1 µmol to a 200 nmol synthesis scale, cleave and deprotect the CAL dye labeled oligo in 200 µL of 2-(2aminoethoxy)-ethanol (Aldrich # A54059) at 60 °C for 2.5 hours. The dye labeled CPG will lose the blue color after the addition of the 2-(2aminoethoxy)-ethanol. Remove the sample from the heat block and cool to approximately 25 °C. Add 1.5 mL of 1,4-dioxane. Shake the tube by hand. Centrifuge at 13,000 RPM for 30 seconds and remove the supernatant with a pipette. Repeat the process with another 1.5 mL of 1.4-dioxane. As the treatment with the 1,4-dioxane removes the base and precipitates out the DNA the sample should regain its blue color. After the removal of the 1,4-dioxane, add 1 ml of water containing 30 µL of acetic acid. CAL Fluor Red 635 will degrade if left in the presence of base for an extended amount of time. The use of strong bases, such as concentrated ammonia, causes degradation of the dye and should be avoided. CAL Fluor Red 635 is light sensitive. Prolonged exposure to light may cause photo bleaching. This product is susceptible to oxidation when left out in the open or undessicated. Unused amidite solution can be stored under argon and its functionality maintained for up to 24 hours. Image of cleaved and deprotected structure:The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is: 761.69 |
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