Ampligase® 10X Reaction Buffer Ampligase DNA连接酶缓冲液
货号：A1905B
规格：5 mL
价格：1034
产品类型：Modifying Enzymes连接酶
品牌：Lucigen
| Ampligase® Thermostable DNA Ligase catalyzes NAD-dependent ligation of adjacent 3´-hydroxylated and 5´-phosphorylated termini in duplex DNA structures that are stable at high temperatures. Derived from a thermophilic bacterium, the enzyme is stable and active at much higher temperatures than conventional DNA ligases. Its half-life is 48 hours at 65°C and greater than 1hour at 95°C. Ampligase DNA Ligase has been shown to be active for at least 500 thermal cycles (94°C/80°C) or 16 hours of cycling.This exceptional thermostability permits extremely high hybridization stringency and ligation specificity. Ampligase DNA Ligase has no detectable activity in ligating blunt-ended DNA and has no activity on RNA or RNA:DNA hybrids.Ampligase®ThermaltableDNA连接酶催化在高温下稳定的双链DNA结构中相邻3'-羟基化和5'-磷酸化末端的NAD依赖性连接。该酶来自嗜热细菌，在比常规DNA连接酶高得多的温度下稳定且有活性。 其半衰期在65°C时为48小时，在95°C时为1小时以上。该扩增酶DNA连接酶在至少500次热循环（94°C / 80°C）或16小时循环中具有活性。这种特殊的热稳定性允许极高的杂交严谨性和连接特异性。该DNA连接酶在连接平端DNA时没有可检测的活性，对RNA或RNA：DNA杂交体没有活性 |
| 优点： |
| ▪High thermostability allows ligation using high-stringency hybridization conditions▪High specificity and stringency permits sensitive detection of SNPs； |
| 相关产品： |
| ▪Ampligase® DNA Ligase Kit（货号#A8101）▪ Ampligase® DNA Ligase（货号#A0110K/A3210K） |
技术参数 产品优点 - High thermostability allows ligation using high-stringency hybridization conditions- High specificity and stringency permits sensitive detection of SNPs
产品应用 - Ligation amplification (Ligase Chain Reaction, LCR) - Repeat Expansion Detection (RED) - Simultaneous mutagenesis of multiple sites - Other ligation-based detection methods - 灵敏检测单核苷酸多态性（SNPs） - 连接扩增（连接酶链式反应、LCR） - Gibson Assembly 克隆 - 重复扩增检测（RED） - 同时突变多个位点
推荐用于 Highly thermostable DNA ligase for applications where ligation at high temperature is beneficial
组成成分 Ampligase 10X Reaction Buffer: 200 mM Tris-HCl (pH 8.3), 250 mM KCl, 100 mM MgCl2, 5 mM NAD, and 0.1% Triton® X-100.
辅助产品 Ampligase® DNA Ligase
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