Ampligase® DNA Ligase Kit/Ampligase DNA连接酶试剂盒
货号：A8101
规格：1000 Units
价格：2768
产品类型：Modifying Enzymes连接酶
品牌：Lucigen
| Ampligase® Thermostable DNA Ligase catalyzes NAD-dependent ligation of adjacent 3´-hydroxylated and 5´-phosphorylated termini in duplex DNA structures that are stable at high temperatures. Derived from a thermophilic bacterium, the enzyme is stable and active at much higher temperatures than conventional DNA ligases. Its half-life is 48 hours at 65°C and greater than 1hour at 95°C. Ampligase DNA Ligase has been shown to be active for at least 500 thermal cycles (94°C/80°C) or 16 hours of cycling.This exceptional thermostability permits extremely high hybridization stringency and ligation specificity. Ampligase DNA Ligase has no detectable activity in ligating blunt-ended DNA and has no activity on RNA or RNA:DNA hybrids.Ampligase热稳定性DNA连接酶来自嗜热细菌，在比常规DNA连接酶高得多的温度下稳定且有活性。该酶催化在高温下稳定的双链DNA结构中相邻的3，-羟基化和5，磷酸化末端的NAD依赖性连接。它在65℃条件下的半衰期为48小时，95℃条件下的半衰期为1小时以上。它至少在500个热循环（94℃/80℃）或16小时循环中具有活性，可实现极高的杂交严密性和连接特异性。它不具有连接平端DNA、RNA或RNA：DNA杂交体的活性。 |
| 优点： |
| ▪High thermostability allows ligation using high-stringency hybridization conditions▪High specificity and stringency permits sensitive detection of SNPs；▪ 高热稳定性允许使用高严格杂交条件进行连接▪高特异性和严谨性允许对SNP进行灵敏检测 |
| 组成成分： |
| ▪Ampligase DNA Ligase▪10X Reaction Buffer▪ Control DNA |
| 相关产品： |
| ▪Ampligase® DNA Ligase （货号#A0110K/A3210K）▪ Ampligase® 10X Reaction Buffer（货号#A1905B） |
技术参数 产品优点 - 高热稳定性允许使用高严格杂交条件进行连接- 高特异性和严谨性允许对SNP进行灵敏检测
产品应用 - Ligation amplification (Ligase Chain Reaction, LCR) - Repeat Expansion Detection (RED) - Simultaneous mutagenesis of multiple sites - Other ligation-based detection methods - 灵敏检测单核苷酸多态性（SNPs） - 连接扩增（连接酶链式反应、LCR） - Gibson Assembly 克隆 - 重复扩增检测（RED） - 同时突变多个位点
产品类型 DNA连接酶试剂盒
推荐用于 高耐热性DNA连接酶，适用于高温连接有优势的应用
组成成分 Ampligase DNA Ligase Kit contains: Ampligase DNA Ligase, 10X Reaction Buffer, and Control DNA.
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