TransforMax™ EPI300™ Electrocompetent E. colis，TransforMax™ EPI300™电感受态大肠杆菌
货号：EC300110,EC300150
规格：20 rxn,100 rxns
价格：4588,19025
产品类型：感受态细胞
品牌：Lucigen
| TransforMax™ EPI300™ E. coli have been specifically engineered for use with Lucigen’s CopyControl™ Cloning Systems.The cells contain a mutant trfA gene, whose protein product is required for initiation of replication from the oriV origin of replication contained on the CopyControl vectors. The trfA gene is under tightly regulated control of an inducible promoter. When grown on standard LB plates or in LB or SOC culture medium, expression of the trfA gene is repressed. Addition of the CopyControl Induction Solution (provided with the cells) induces expression of the trfA gene and subsequent utilization of the oriV origin of replication and high copy amplification of the CopyControl fosmid clones. |
| 优点： |
| ▪ Clone large inserts and transform large plasmids - up to at least 145 kb plasmid DNA.▪ Generate clones with inducible copy number using CopyControl™ vectors.▪ Achieve high transformation efficiencies: >1 × 1010 cfu/µg pUC19 DNA.▪ Increase Gibson Assembly® colony counts with high-efficiency electroporation |
技术参数 产品优点 - Clone large inserts and transform large plasmids - up to at least 145 kb plasmid DNA. - Generate clones with inducible copy number using CopyControl™ vectors. - Achieve high transformation efficiencies: >1 × 1010 cfu/µg pUC19 DNA. - Increase Gibson Assembly® colony counts with high-efficiency electroporation
产品应用 - Mutant trfA gene under tightly regulated control of an inducible promoter for complete copy number control of CopyControl clones. - Supports blue/white screening of vectors expressing the LacZ′ α-complementing peptide. - Restriction minus for efficient cloning of methylated genomic (e.g. mammalian genomic) DNA. - Endonuclease minus (endA1) to ensure high yields of clones. - Recombination minus (recA1) to ensure the stability of large cloned inserts.
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