GATA6 Polyclonal Antibody

货号： PA1-104X ,PA1-104

规格：20 µL,100 µg

价格：2148,5468

产品类型：免疫组化一抗

品牌：eBioscience

物种：人/小鼠

宿主：兔

抗体亚型：IgG

荧光染料：Purified

类型:	多抗	同型对照：
浓度：	1 mg/mL	用法：	1：200 (ICC)；1：200 (IF)；1:50-1:100（IHC (P)）；1;1000 (WB)
免疫原性：	Synthetic peptide sequence KYSGQDGLYIGVSLASPAE and KRFGAAGADASDSRAFPAR

靶标信息This gene encodes a member of the GATA family of zinc-finger transcription factors that are named for the consensus nucleotide sequence they bind in the promoter regions of target genes. GATA6 is thought to be important for regulating terminal differentiation and/or proliferation and plays a critical role in endoderm development.

数据

GATA6 Antibody (PA1-104X) in IF Immunofluorescent analysis of GATA6 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 5% normal goat serum (Product # 31873) for 15 minutes at room temperature. Cells were then probed with a rabbit polyclonal antibody recognizing GATA6 (Product # PA1-104) at a dilution of 1:200 for at least 1 hour at 37°C. Cells were then washed with PBS and incubated with DyLight 488 goat-anti-rabbit secondary antibody at a dilution of 1:200 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan at 10X magnification. GATA6 Antibody (PA1-104X) in IHC (P) Immunohistochemistry was performed on human stomach tissue. To expose target protein, antigen was retreived using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a GATA6 Rabbit polyclonal antibody (Product # PA1-104) at a dilution of 1:100 overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 40X magnification.

二抗荧光二抗免疫组化一抗标签抗体

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