GATA4 Polyclonal Antibody
货号： PA1-102 ,PA1-102X
规格：100 µg,20 µL
价格：5468,2148
产品类型：免疫组化一抗
品牌：eBioscience
物种：人/小鼠
宿主：兔
抗体亚型：IgG
荧光染料：Purified
类型: | 多抗 | 同型对照： | |
浓度： | 1 mg/mL | 用法： | 3-5 µg/1x10^6 cells（Flow）；1：50-1：200 (ICC)；1：50-1：200 (IF)；1:100-1:1000（IHC (P)）；1：500-1：2000 (WB) |
免疫原性： | Pooled peptides: RKEGIQTRKRKPKNLNKSK and CKQDSWNSLVLADSHGDIITA |
产品详细信息
PA1-102 has been successfully used in Western blot, FACS, immunohistochemistry (paraffin) and immunofluorescence applications with human and mouse samples. PA1-102 can be used for immunofluorescence analysis of GATA4 in the endoderm derived from human iPSCs.
靶标信息
GATA binding factor 4 (GATA4) is a GATA family transcription regulator involved in embryonic development. In addition, members of this family recognize the GATA motif which is present in the promoters of many genes. GATA4 is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in this gene have been associated with cardiac septal defects.
数据 |
| GATA4 Antibody (PA1-102) in Flow Flow cytometry analysis of GATA4 in NIH/3T3 cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were penetrated by dropping the supernatant, adding 90% methanol and incubated for 10 minutes at room temperature. Follwing penetration, cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a GATA4 polyclonal antibody (Product # PA1-102) at a dilution of 2 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-rabbit IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis. |
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| 二抗荧光二抗免疫组化一抗标签抗体 |
| 参考文献： |
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