Basic Fibroblast Growth Factors (bFGF), Human Recombinant

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Basic Fibroblast Growth Factors (bFGF), Human Recombinant

货号:354060

规格:10µg, 1/Pack

价格:321

产品类型:多能干细胞培养

品牌:Corning

Basic Fibroblast Growth Factor (bFGF), human recombinant, 10µg, is a heparin-binding mitogenic protein that enhances the proliferation of a wide variety of cell types under serum-free or serum-reduced conditions. 1/pack, formerly Cat. #40060. These growth factors have been used to promote long-term proliferation in cell culture and to induce cell differentiation in a variety of cell types under serum-free or reduced serum conditions.FormulationLyophilized from phosphate buffered saline, carrier-free; reconstitute in dH2O or serum-free mediumPreparation and StorageStable for at least three months at -20°C (lyophilized) or = one month solubilized at -20°C. Do not store in frost-free freezer. Avoid multiple freeze/thaws.Quality
  • Purity: >95% by SDS-PAGE
  • Tested for ability to promote proliferation of FBHEC
  • Filtered (0.2 µm membrane) and tested and found negative for bacteria, fungi and mycoplasma
  • Recombinant, E. coli

Biocoat hES细胞培养系统可提供无需滋养层辅助的培养干细胞表面—康宁Matrigel基质,它是从EHS(Engelbreth-Holm-Swarm)小鼠肉瘤中分离得到的可溶性再生基底膜,可预包被在培养器皿表面,替代传统的细胞滋养层。mTeSRI培养基是干细胞培养配套的无血清培养基,配合hES细胞专用康宁Matrigel细胞基质,可用于hES的扩增和维持hES的未分化状态。另一个可选的是康宁高浓度层粘连蛋白/巢蛋白混合物包被的细胞培养表面,其担保纯度高达90%,专门用于支持hES细胞的生长。但不同于hES Matrigel细胞基质,它不能维持hES细胞的未分化状态。
在Matrigel基质上培养的hES细胞具有以下特点:
- 维持正常的染色体组型;- 表现稳定的增殖率和高端粒酶活性;- 表达特征性的未分化hES细胞标记物;- 在小鼠体内可分化成三胚层细胞

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技术参数
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