Pierce Strong Cation Exchange Spin Column, Mini 阳离子交换离心柱
货号：90008
规格：24 columns
价格：3597
产品类型：蛋白提取/纯化
品牌：Thermo Fisher
| Thermo Scientific Pierce Strong Cation Exchange Spin Column, Mini enable rapid protein fractionation and sample preparation based on charge differences.Pierce Strong Ion Exchange Spin Columns use membrane-adsorption as a chromatographic method to fractionate proteins based on their charge differences. The matrix has a highly porous structure with pores larger than 3000nm, providing proteins easy access to the membrane's charged ligands. Therefore, adsorptive membranes maintain high efficiencies at high-flow rates and when fractionating large biomolecules with small diffusivities.Ion exchange chromatography separates molecules based on differences in their accessible surface charges. This technique is widely used in the pre-fractionation or purification of a target protein from crude biological samples. Novel membrane-based ion exchange chromatography is attracting attention because of its advantages over resin-based column chromatography. Membrane-based ion exchange chromatography has shorter diffusion times than resin-based chromatography. Interactions between molecules and active sites on the membrane occur in a convective manner through pores, which shortens the diffusion time compared with fluid inside the pores of a resin particle. Also, the relatively mild binding and eluting conditions of this separation method produce high protein recovery with intact biological activity. |
| 特点： |
| - Fast and simple—membrane-based spin format eliminates column packing- Convenient and expandable—centrifugal format enables convenient processing of multiple samples in parallel- Robust—membrane adsorber spin columns do not crack or run dry- Low bed volume—small membrane adsorber bed volumes make working with low buffer volumes possible, leading to concentrated elution fractions |
| 应用： |
| - Pre-fractionation of proteins in lysate- Scouting purification conditions for new protein preparation protocols- Removal of endotoxins from monoclonal antibodies- Polishing His-tagged proteins after metal chelate chromatography- Purification and concentration of proteins- Purification of antibodies from serum, ascites or tissue culture supernatant- Removal of detergent from protein solutions- Sample preparation before 1D or 2D PAGE- Purification of phosphopeptides before MS analysis |
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