Pierce Streptavidin Agarose Columns, 1 mL 链霉亲和素琼脂糖柱
货号：20351
规格：5 columns
价格：7155
产品类型：蛋白提取/纯化
品牌：Thermo Fisher
| Thermo Scientific Pierce Streptavidin Agarose Columns are 5-mL capacity columns containing 1 mL streptavidin agarose, for use in a variety of biotin-binding affinity purification methodsPierce Streptavidin Agarose consists of purified recombinant streptavidin that has been covalently immobilized onto high-quality crosslinked 6% beaded agarose. Streptavidin is a tetrameric biotin-binding protein. The agarose beads have physical and chemical properties that enable them to be used in a variety of batch- or column-type affinity procedures. Biotinylated antibodies, proteins, peptides, nucleic acids and other molecules or interaction complexes can be captured, immunoprecipitated, removed or purified from samples using this streptavidin resin.Streptavidin is a tetrameric protein containing four biotin-binding sites similar to avidin. The native protein from Streptomyces avidinii is carbohydrate-free, has an acidic pI of 5.5 and a mass of 75 kDa. The recombinant protein used to manufacture Thermo Scientific Pierce Streptavidin Resins has a near-neutral isoelectric point and a mass of 53kDa. Streptavidin generally has less nonspecific binding than avidin (from chicken egg white) because of the absence of carbohydrates. |
| 特点： |
| - Streptavidin—purified recombinant streptavidin protein (53kDa, near-neutral pI); tetrameric with four biotin-binding site per molecule- Agarose resin—support is crosslinked 6% beaded agarose (CL-6B), the most popular resin for protein affinity purification methods- Inert and stable—streptavidin is immobilized by charge-free, leach-resistant covalent bonds, resulting in low nonspecific binding and enabling multiple uses without decline in yield- Standard capacity—this variety of beads has a normal load of immobilized streptavidin, providing a binding capacity of 1 to 3 mg of biotinylated BSA per mL of resin |
| 应用： |
| - Purification of membrane antigens in conjunction with biotinylated monoclonal antibodies- Isolation of cell-surface proteins that have been labeled with a cleavable biotinylation reagent- Isolation of cell-surface glycoproteins using biotinylated Concanavalin A- Recovery of single-stranded biotinylated DNA for dideoxy sequencing- Streptavidin-coated plates used for direct solid-phase sequencing- In vitro mutagenesis with streptavidin-coated plates- DNA fingerprinting with streptavidin-coated plates |
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