Pierce Fast Western Blot Kit, ECL Substrate 快速蛋白质印迹试剂盒，ECL底物
货号：35050,35055
规格：1 L,200 mL
价格：4261,2061
产品类型：免疫印迹/组化
品牌：Thermo Fisher
| The Thermo Scientific Pierce Fast Western Blot Kit, ECL Substrate contains optimized reagents that shorten the time required to perform a typical chemiluminescent Western blot from 4 hours down to approximately 55 minutes.The kit provides all the reagents necessary to complete a Western blot being probed with a mouse or rabbit primary antibody. The protocol requires minimal hands-on time and yields results comparable to classic Western blotting with ECL. The included Pierce ECL Substrate produces a chemiluminescent signal, which is detected using photographic or other imaging methods. Blots can be repeatedly exposed to film to obtain optimal results or stripped of the primary antibody and immunodetection reagents and reprobed.The Pierce Fast Western Blot Kit is reagent-based system that provides optimized reagents for blocking, antibody dilution and detection of Western blots with Pierce ECL substrate. The protocol is a quick, efficient and economic way to obtain Western blot results without the hassle of buying an instrument and the consumables required by other popular rapid Western blotting systems. No vacuum pump is needed so there are never any clogged lines or membranes that can occur when using instrument-based systems. Because there is no apparatus, there is no need to buy disposable blotting trays that limit the number of blots processed at one time. |
| 特点： |
| - Fast—all the sensitivity of Pierce ECL substrate and saving 4 to 5 hours per blot- Convenient—no expensive hardware or vacuum required; no clogging issues- Simple—optimized protocol makes Western blot analysis easier than ever- Economical—cost as little as $8 per blot; no expensive consumables or extra equipment- Excellent stability—kit is stable for 1 year stored at 4°C- Easy—complete kit gives you all the components you need to block and probe and develop a blot with your mouse or rabbit primary antibody |
| 数据： |
Comparable results obtained with classic Western blotting protocol and Fast Western Blot KitThe detection sensitivity for various target proteins was compared using the Thermo Scientific Pierce Fast Western Blot Kit and the classical Western blot protocol. Panels A and B contained 10, 2, 0.4 and 0.08µg of protein from A549 cell lysates in lanes 1-4, respectively.Panels C and D contained 2, 1, 0.5, 0.25 and 0.125µg of 293T cell lysate in lanes 1-5 of Panel C and 25, 12.5, 6.25, 3.1 and 0.39µg in lanes 1-5 of Panel D.Protein detection: Panel A. Mouse anti-Cyclin D1 antibody (BD Pharmingen) on PVDF.Panel B. Rabbit anti-Beta Catenin antibody (LabVision) on PVDF.Panel C. Mouse anti-GAPDH antibody (Millipore) on nitrocellulose.Panel D. Rabbit anti-Cdk2 antibody (Upstate) on PVDF. |
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