3'-DMT-dC(Ac)-Suc-CPG; 1000 Å
货号：BG1-1100i-1
规格：1 g
价格：1055
产品类型：核酸合成
品牌：LGC Biosearch Technologies
| 3'-DMT-dC(Ac)-Suc-CPG; 1000 Å is an inverse linkage CPG used to create a 3'-3' linkage on the end of an oligonucleotide. An inverted 3'-3' linkage is desirable when needing to block polymerase extension through the 3'-terminus of a PCR probe, additionally oligonucleotides with inverted 3'-linkages are used for some sequencing techniques. |
| 特性： |
| - Chemical Name: 3'-DMT-dC(Ac)-Suc-CPG; 1000 Å- Appearance: White Powder- Extinction Coefficient at 260 nm: 7050 |
| 产品用途： |
| - Protecting group deblock conditions: 3'DMT groups detritylate slowly. Increase detritylation time by 50%.- Cleavage conditions: Use concentrated ammonia for 90 minutes at 25 °C when using standard amidites or 1:1 ammonia:methylamine (AMA) for 25 minutes at 25 °C when using fast deprotecting amidites.- Deprotection conditions: When using fast deprotecting amidites (eg. C-Ac, G-DMF) use concentrated ammonia for 1 hour or AMA for 30 minutes at 60 °C. When using standard amidites (eg. C-Bz, G-iBu) use concentrated ammonia for 5 hours at 60 °C.- The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is: 289.18 |
| 数据： |
| |
QQ:1749072012
