5'-DMT-dG(DMF)-Suc-CPG; 500 Å (High Load)
货号:BG5-1200FH-1,BG5-1200FH-10,BG5-1200FH-100
规格:1 g,10 g,100 g
价格:633,5628,60149
产品类型:核酸合成
品牌:LGC Biosearch Technologies
Biosearch Technologies high loaded 5'-DMT-dG(DMF)-Suc-CPG is used for the placement of dG on the 3' end of oligonucleotides. The high loaded 500 Å support is useful for the synthesis of oligos up to 25-mers in length, especially when larger amounts of product are desired. The 500Å support allows for greater nucleoside loading than do supports with larger pore sizes. This high loaded support has a fast deprotecting group making it suitable for use with base sensitive chemistries. |
特性: |
- Appearance: white powder- Extinction Coefficient at 260 nm: 12010 |
用途: |
- Cleavage conditions: Use concentrated ammonia for 90 minutes at 25 °C when using standard amidites or 1:1 ammonia:methylamine (AMA) for 25 minutes at 25 °C when using fast deprotecting amidites.- Deprotection conditions:When using fast deprotecting amidites (eg. C-Ac, G-DMF) use concentrated ammonia for 1 hour or AMA for 30 minutes at 60 °C. When using standard amidites (eg. C-Bz, G-iBu) use concentrated ammonia for 5 hours at 60 °C. Image of cleaved and deprotected structure: - The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is: 329.2 |
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