ROX-CPG (5'-DMT-mdC(TEG-ROX)-Phos-CPG); 500 Å
货号：BG5-5021-1,BG5-5021-100
规格：1 g,100 mg
价格：22512,2462
产品类型：核酸合成
品牌：LGC Biosearch Technologies
| Biosearch Technologies' 5'-DMT-mdC(TEG-ROX)-Phosphate CPG is based on a modified cytosine residue linked to the ROX moiety via a triethyleneglycol spacer. The 3' end is conjugated to the CPG support via phosphate linkage. Our ROX CPG support allows for the introduction of a reporter molecule onto the 3'-terminus end of the oligonucleotide. |
| 特性： |
| - Appearance:purple powder- Absorption Maximum (Lambda Max): 575- Extinction Coefficient at Lambda max:91000- Extinction Coefficient at 260 nm:38500- Fluorescence Maximum: 602 |
| 用途： |
| - Synthesis conditions: Follow the instrument manufacturer's protocol, with noted changes, when using this product. Synthesis can be performed on these supports according to standard procedures with deprotection and cleavage modifications noted.- Cleavage conditions: Use t-butylamine/water (1:3) for 45 minutes at 25 °C. The use of ammonia must be avoided, as it will cause degradation of the ROX moiety.- Deprotection conditions: When using fast deprotecting amidites (eg. C-Ac, G-DMF, G-PAC) use t-butylamine/Water (1:3) for 6 hours at 65° C. When using standard amidites (eg. C-Bz, G-iBu) use t-butylamine/Water (1:3) overnight (12-15 hours) at 65°C. The use of ammonia must be avoided, as it will cause degradation of the ROX moiety. Image of cleaved and deprotected structure: - The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is: 1103.06 |
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