DABCYL-Suc-CPG (5'-DMT-mdC(TEG-DABCYL)-Suc-CPG); 500 Å

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DABCYL-Suc-CPG (5'-DMT-mdC(TEG-DABCYL)-Suc-CPG); 500 Å

货号:BG5-5025S-1,BG5-5025S-100

规格:1 g,100 mg

价格:11256,1407

产品类型:核酸合成

品牌:LGC Biosearch Technologies

Biosearch Technologies’ 5'-DMT-mdC(TEG-DABCYL)-Suc CPG is based on a modified cytosine residue linked to the Dabcyl chromophore via a triethyleneglycol spacer. The 3' end is conjugated to the CPG support via succinyl linkage. 5’-DMT-mdC(TEG-DABCYL)-Suc-CPG is specifically suited for the preparation of molecular beacons or fluorescent energy transfer probes. DABCYL is used as a quencher for fluorophore groups such as fluorescein, TAMRA and JOE.
特性:
- Appearance: orange powder- Absorption Maximum (Lambda Max): 472- Extinction Coefficient at Lambda max:32000- Extinction Coefficient at 260 nm: 14333
用途:
- Synthesis conditions:Follow the instrument manufacturer's protocol when using this product.- Cleavage conditions:Use concentrated ammonia for 90 minutes at 25 °C.- Deprotection conditions:If using fast deprotecting amidites (eg. C-Ac, G-DMF, G-PAC)) deprotect in concentrated NH4OH for 1 hour at 60 °C. For standard amidites (eg. C-Bz, G-iBu) deprotect in concentrated NH4OH for 5 hours at 60 °C. If using base sensitive fluorophores (ie. TAMRA or Rhodamine based dyes) we recommend deprotecting oligonucleotide in t-butylamine/H2O (1:3) for 12 hours at 60 °C- The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is: 757.77
数据:

技术参数
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