BHQ-1 T Linker Arm, CPG; 500 Å
货号：BG5-5041TN-1,BG5-5041TN-100
规格：1 g,100 mg
价格：35175,4221
产品类型：核酸合成
品牌：LGC Biosearch Technologies
| BHQ®-1 T-Linker 500Å CPG is used to add the non-fluorescent quencher (BHQ-1) to the 3' end of an oligonucleotide. BHQ-1 quencher has maximal absorption in the 480 to 580 nm range, which provides excellent quenching of fluorophores emitting in this range, such as FAM, TET, JOE, and HEX. The BHQ-1 T-Linker Arm CPG has a 3' phosphate followed by a glycolate linkage to the CPG support, which allows for rapid cleavage of the oligo from the support and is labile enough for base-sensitive oligonucleotides. The 500 Å CPG is useful for the synthesis of oligos up to 50-mers in length, especially when larger amounts of product are desired, as it allows for greater nucleoside loading amounts than do supports with larger pore sizes. |
| 特性： |
| - Appearance: dark red powder- Absorption Maximum (Lambda Max): 548- Extinction Coefficient at Lambda max: 41600- Extinction Coefficient at 260 nm: 30100 |
| 用途： |
| - Synthesis conditions: Follow the instrument manufacturer's protocol when using this product.- Cleavage conditions: When using fast deprotecting amidites use concentrated Ammonia for 20 min at 25 °C or Ammonia/ Methylamine (AMA) (1:1) for 5 min at 25 °C; or
t-butylamine/H2O (1:3) for 45 min at 25 °C (TAMRA requires deprotection with TBA). When using standard amidites (eg. C-Bz, G-iBu) use concentrated Ammonia for 20 min at 25 °C.- Deprotection conditions: When using fast deprotecting amidites (eg. C-Ac, G-DMF) deprotect in concentrated ammonia for 1 hour or AMA for 30 minutes at 60 °C. When using standard amidites (eg. C-Bz, G-iBu) deprotect in concentrated ammonia for 5 hours at 60 °C. If you are using base sensitive fluorophores (ie. TAMRA) we recommend deprotecting the oligonucleotide in t-butylamine/H2O (1:3) for 6 hours at 60 °C. - The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is: 1098.98 |
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