BHQ-1 CPG; Glycolate, 500 Å
货号：BG5-5041G-1,BG5-5041G-100
规格：1 g,100 mg
价格：21105,2673
产品类型：核酸合成
品牌：LGC Biosearch Technologies
| BHQ®-1 Glycolate CPG is used to add the non-fluorescent quencher, BHQ-1 dye to the 3' end of an oligonucleotide. BHQ-1 quencher has maximal absorption in the 480 to 580 nm range, which provides excellent quenching of fluorophores emitting in this range, e.g. FAM, TET, JOE, HEX, etc. BHQ-1 Glycolate CPG has a glycolate linkage to the CPG support. The glycolate linker allows for rapid cleavage of the oligonucleotides and is labile enough for base sensitive oligonucleotides. The 500 Å CPG is useful for the synthesis of oligos up to 50 bases in length, especially when larger amounts of product are desired, as it allows for greater nucleoside loading amounts than do supports with larger pore sizes. |
| 特性： |
| - Chemical Name: BHQ-1 CPG; Glycolate, 500 Å- Appearance: dark red powder- Absorption Maximum (Lambda Max): 534- Extinction Coefficient at Lambda max: 34000- Extinction Coefficient at 260 nm: 8000 |
| 用途： |
| - Cleavage conditions:When using fast deprotecting amidites use concentrated Ammonia for 45 min at 25 °C or Ammonia/ Methylamine (AMA) (1:1) for 15 min at 25 °C; or
t-butylamine/H2O (1:3) for 90 min at 25 °C (TAMRA requires deprotection with TBA). When using standard amidites (eg. C-Bz, G-iBu) use concentrated Ammonia for 45 min at 25 °C.- Deprotection conditions:When using fast deprotecting amidites (eg. C-Ac, G-DMF, G-PAC) deprotect in concentrated ammonia for 1 hour or AMA for 30 minutes at 60 °C. When using standard amidites (eg. C-Bz, G-iBu) deprotect in concentrated ammonia for 5 hours at 60 °C. If you are using base sensitive fluorophores (ie. TAMRA) we recommend deprotecting the oligonucleotide in t-butylamine/H2O (1:3) for 12-15 hours at 60 °C. Image of cleaved and deprotected structure: - The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:554.49 |
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