CEA Human ELISA Kit/人CEA ELISA试剂盒
货号：EHCEA,EHCEAX5,EHCEAX10
规格：96 tests,5X96 tests,10X96 tests
价格：7311,29891,52892
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 200 pg/mL |
| 检测范围 | 0.343-250 ng/mL |
样本类型/体积 | 上清液 100 μL 血清 50 μL 血浆 50 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | CD66e (CEACAM5) |
| Protein aliases | Carcinoembryonic antigen, CEA, Meconium antigen 100, Carcinoembryonic antigen-related cell adhesion molecule 5, CD66e |
物种（Tested） | 人 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | CD66e, CEA, CEACAM5 |
| Gene ID | (Human) 1048 |
| Gene symbol | CEACAM5 |
| UniProt ID | (Human) P06731 |
| The Human Carcino Embryonic Antigen (Hu CEA) ELISA quantitates Hu CEA in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu CEA.人Carcino胚胎抗原（Hu CEA）ELISA可以定量人血清，血浆或细胞培养基中的Hu CEA。 该检测试剂盒将专门识别天然和重组Hu CEA。Principle of the method 方法原理The Human CEA solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人CEA固相夹心ELISA（酶联免疫吸附测定）是测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息CEA (Carcino Embryonic Antigen, CD66e) is synthesized during development in the fetal gut, and re-expressed in increased amounts in intestinal carcinomas and several other tumors. CEA is a member of carcinoembryonic antigens, immunoglobulin supergene family and consists of a single N domain (structural homology to the immunoglobulin variable) and six immunoglobulin constant-like A (A1, A2, A3) and B domains (B1, B2, B3). Antibodies to CEA are useful in identifying the origin of various metastatic adenocarcinomas and in distinguishing pulmonary adenocarcinomas (60 to 70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). CEA is a member of a large family of glycoproteins, a useful tumor marker for adenocarcinoma, and found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon. Two subgroups of the CEA family, the CEA cell adhesion molecules and the pregnancy-specific glycoproteins, are located within a 1.2 Mb cluster on the long arm of chromosome 19. Eleven pseudogenes of the CEA cell adhesion molecule subgroup are also found in the cluster. CEA was originally described in bile ducts of liver as biliary glycoprotein. Subsequently, CEA was found to be a cell-cell adhesion molecule detected on leukocytes, epithelia, and endothelia. The encoded protein mediates cell adhesion via homophilic as well as heterophilic binding to other proteins of the subgroup. Multiple cellular activities have been attributed to the encoded protein, including roles in the differentiation and arrangement of tissue three-dimensional structure, angiogenesis, apoptosis, tumor suppression, metastasis, and the modulation of innate and adaptive immune responses. Multiple transcript variants encoding different isoforms have been reported, but the full-length nature of all variants has not been defined.CEA（Carcino Embryonic Antigen，CD66e）是在胎儿肠道发育过程中合成的，并在肠癌和其他几种肿瘤中重新表达。 CEA是癌胚抗原（免疫球蛋白超基因家族）的成员，由一个N结构域（与免疫球蛋白变量具有结构同源性）和六个免疫球蛋白常数样A（A1，A2，A3）和B结构域（B1，B2，B3）组成。 CEA抗体可用于识别各种转移性腺癌的起源，还可用于区分胸膜间皮瘤（罕见或弱于CEA +）的肺腺癌（60％至70％为CEA +）。 CEA是糖蛋白大家族的成员，糖蛋白是腺癌的有用的肿瘤标志物，并且存在于真皮内衍生的消化系统上皮和胎儿结肠的腺癌中。 CEA家族的两个亚组，即CEA细胞粘附分子和妊娠特异性糖蛋白，位于19号染色体长臂的1.2 Mb簇中。在该簇中还发现了11个CEA细胞粘附分子亚组的假基因。 CEA最初在肝胆管中被描述为胆汁糖蛋白。随后，发现CEA是在白细胞，上皮和内皮上检测到的细胞粘附分子。编码的蛋白通过与亚组其他蛋白的同种和异种结合介导细胞粘附。多种细胞活性已经归因于编码的蛋白质，包括在组织三维结构的分化和排列，血管生成，细胞凋亡，肿瘤抑制，转移以及固有和适应性免疫应答的调节中的作用。已经报道了编码不同同工型的多个转录物变体，但是尚未定义所有变体的全长性质。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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