NOV Human ELISA Kit/人NOV ELISA试剂盒
货号：EHNOV,EHNOVX5,EHNOVX10
规格：96 tests,5X96 tests,10X96 tests
价格：7238,29826,52892
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 2 pg/mL |
| 检测范围 | 0.041-30 ng/mL |
样本类型/体积 | 上清液 100 μL 血清 50 μL 血浆 50 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | NOV |
| Protein aliases | CCN family member 3, Cellular communication network factor 3, IBP-9, IGF-binding protein 9, Insulin-like growth factor-binding protein 9, Nephro blastoma-overexpressed gene protein homolog, nephroblastoma-overexpressed gene protein homolog, NovH, Protein NOV homolog |
物种（Tested） | 人 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | CCN3, IBP-9, IGFBP-9, IGFBP9, NOV, NOVH |
| Gene ID | (Human) 4856 |
| Gene symbol | NOV |
| UniProt ID | (Human) P48745 |
| The Human NOV ELISA quantitates Hu NOV in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu NOV.人NOV ELISA可以定量人血清，血浆或细胞培养基中的人NOV。 该检测试剂盒将只识别天然和重组的人 NOV。Principle of the method 方法原理The Human NOV solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人NOV固相夹心ELISA（酶联免疫吸附测定）设计用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息The CCN (CTGF/Cyr61/NOV) family of genes presently consists of six distinct members, which encode proteins that participate in fundamental biological processes such as cell proliferation, adhesion, migration, differentiation, wound healing, angiogenesis and several pathologies including fibrosis and tumorigenesis. Whereas Cyr61 and CTGF act as positive regulators of cell growth, nov (nephroblastoma overexpressed, CCN3 or NOVH) provides the first example of a CCN protein with negative regulatory properties and the first example of aberrant expression being associated with tumor development. In animals and humans increased expression of nov is detected in tissues where calcium is a key regulator, such as the adrenal gland, central nervous system, bone and cartilage, heart muscle and kidney. The nov protein associates with Notch1 extracellular domain and inhibits myoblast differentiation via Notch signaling pathway.CCN（CTGF / Cyr61 / NOV）基因家族目前由六个不同的成员组成，这些成员编码参与基本生物学过程（例如细胞增殖，粘附，迁移，分化，伤口愈合，血管生成和包括纤维化和肿瘤发生在内的几种病理学）的蛋白质 。 Cyr61和CTGF是细胞生长的正调节剂，而nov（肾母细胞瘤过度表达，CCN3或NOVH）则提供了具有负调节特性的CCN蛋白的第一个例子，而异常表达的第一个例子与肿瘤的发展有关。 在动物和人类中，在以钙为主要调节因子的组织（如肾上腺，中枢神经系统，骨骼和软骨，心肌和肾脏）中检测到nov的表达增加。 nov蛋白与Notch1胞外域相关联，并通过Notch信号通路抑制成肌细胞分化。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-20390.htm
QQ:1749072012