HSPA4 (HSP70) Human ELISA Kit/人HSPA4 (HSP70) ELISA试剂盒
货号：EHHSPA4,EHHSPA4X5,EHHSPA4X10
规格：96 tests,5X96 tests,10X96 tests
价格：7311,30065,52892
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 2 ng/mL |
| 检测范围 | 2.469-600 ng/mL |
样本类型/体积 | 上清液 100 μL 血清 50 μL 血浆 50 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | HSPA4 |
| Protein aliases | epididymis secretory sperm binding protein Li 5a, Heat shock 70 kDa protein 4, Heat shock 70-related protein APG-2, heat shock 70kD protein 4, heat shock 70kDa protein 4, heat shock protein, 110 kDa, hsp70 RY, HSP70RY |
物种（Tested） | 人 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | APG-2, APG2, HEL-S-5a, HS24/P52, hsp70, hsp70RY, HSPA4, HSPH2, RY |
| Gene ID | (Human) 3308 |
| Gene symbol | HSPA4 |
| UniProt ID | (Human) P34932 |
| The Human HSPA4 (HSP70) ELISA quantitates Hu HSPA4 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu HSPA4.人HSPA4（HSP70）ELISA定量了人血清，血浆或细胞培养基中的人HSPA4。 该检测试剂盒将专门识别天然和重组的人HSPA4。Principle of the method 方法原理The Human HSPA4 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人HSPA4固相夹心ELISA（酶联免疫吸附测定）设计用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息The HSP 70 family is composed of four highly conserved proteins: HSP 70, HSC 70, GRP 75 and GRP 78. HSP 70 expression is strongly induced in response to heat stress. HSP 70 and HSC 70 play key roles in the cytosolic endoplasmic reticulum and mitochondrial import machinery and are found in both the cytosol and nucleus of mammalian cells. GRP 78 is localized in the endoplasmic reticulum, where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains. GRP 75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. It has been postulated that members of the HSP 70 family act as force-generating motors, relying on the hydrolysis of ATP for their activity.HSP 70家族由四个高度保守的蛋白质组成：HSP 70，HSC 70，GRP 75和GRP78。HSP70的表达强烈响应热应激。 HSP 70和HSC 70在胞质内质网和线粒体输入机制中起关键作用，并且在哺乳动物细胞的细胞质和细胞核中都有发现。 GRP 78位于内质网中，在那里它接收输入的分泌蛋白，并参与新生肽链的折叠和易位。 GRP 75表达仅限于线粒体基质，并有助于核和线粒体来源的新生多肽链的移位和折叠。 据推测，HSP 70家族的成员可以依靠ATP的水解来发挥作用，从而起到发力的作用。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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