CD14 Monoclonal Antibody (Sa2-8), FITC, eBioscience™

货号：11-0141-81,11-0141-82,11-0141-85

规格：50 μg,100 μg,500 μg

价格：925,1721,2934

产品类型：流式抗体

品牌：eBioscience

抗原：CD14

物种：人/小鼠

宿主：大鼠

抗体亚型：IgG

克隆号：Sa2-8

荧光染料：FITC

抗体类型:	流式抗体	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience™
浓度：	0.5 mg/mL	用法：	1 µg/test (Flow)

产品详细信息Description: The Sa2-8 monoclonal antibody reacts with mouse CD14, a 53-55 kDa GPI-linked glycoprotein. CD14 is a receptor for the complexes of LPS and LBP (LPS-Binding Protein) and is shown to associate with Toll-Like Receptor 4 (TLR4) and participate in the signaling and cellular response to bacterial LPS. In mouse, CD14 is expressed on the surface of macrophages and under certain conditions is also found in the serum in a secreted form. Sa2-8 has weak antagonistic activity (in NF-kappaB activation or TNF alpha production with LPS stimulation).Applications Reported: The Sa2-8 antibody has been reported for use in flow cytometric analysis.Applications Tested: This Sa2-8 antibody has been tested by flow cytometric analysis of thioglycolate-elicited peritoneal exudate cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered. 靶标信息CD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils. CD14 also serves as a multifunctional lipopolysaccharide receptor, and is released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 can discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. Further, CD14 has been shown to bind apoptotic cells, and can affect allergic, inflammatory and infectious processes. Alternative splicing results in multiple transcript variants encoding the same CD14 isoform. Diseases associated with CD14 dysfunction include mycobacterium chelonae infection and Croup.

数据

CD14 Antibody (11-0141-81) in Flow Staining of mouse thioglycolate-elicited peritoneal exudate cells with 0.5 µg of Rat IgG2a K Isotype Control FITC (Product # 11-4321-42) (blue histogram) or 0.5 µg of Anti-Mouse CD14 FITC (purple histogram). Total viable cells were used for analysis. CD14 Antibody (11-0141-81) Endocrinology 2016 -Published figure using CD14 monoclonal antibody (Product # 11-0141-81)

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参考文献：

1. Frontiers in molecular neuroscience Increased White Matter Inflammation in Aging- and Alzheimer's Disease Brain. 2. Oncology letters Butyrate upregulates the TLR4 expression and the phosphorylation of MAPKs and NK-κB in colon cancer cell in vitro. 3. International journal of molecular medicine TIM‑4 blockade of KCs combined with exogenous TGF‑β injection helps to reverse acute rejection and prolong the survival rate of mice receiving liver allografts. 4. Nature communications Construction of developmental lineage relationships in the mouse mammary gland by single-cell RNA profiling. 5. Endocrinology T3 Regulates a Human Macrophage-Derived TSH-β Splice Variant: Implications for Human Bone Biology.

技术参数

产品应用 Flow;ICC;IF;FN;WB

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