Periostin (Postn) Mouse ELISA Kit/小鼠骨膜素 (Postn) ELISA试剂盒
货号：EMPOSTN,EMPOSTNX5,EMPOSTNX10
规格：96 tests,5X96 tests,10X96 tests
价格：7238,29891,52892
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：小鼠
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 0.05 ng/mL |
| 检测范围 | 0.66-16 ng/mL |
样本类型/体积 | 上清液 100 μL 血清 2 μL 血浆 2 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | Periostin |
| Protein aliases | OSF-2, osteoblast specific factor 2 (fasciclin I-like), Osteoblast-specific factor 2, Periostin, periostin-like factor, PN |
物种（Tested） | 小鼠 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | A630052E07Rik, AI747096, OSF-2, Osf2, peri, PLF, PN, Postn |
| Gene ID | (Mouse) 50706 |
| Gene symbol | Postn |
| UniProt ID | (Mouse) Q62009 |
| The Mouse Periostin (Postn) ELISA quantitates Ms Periostin in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Periostin.小鼠骨膜素（Postn）ELISA定量检测小鼠血清，血浆或细胞培养基中的骨膜素。 该检测试剂盒将专门识别天然和重组M. 骨膜素。Principle of the method 方法原理The Mouse Periostin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.小鼠Periostin固相夹心ELISA（酶联免疫吸附测定）是测量匹配的抗体对之间结合的靶标量。 已在孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Periostin (PN), also designated osteoblast-specific factor 2 (OSF-2), is a disulfide linked protein originally isolated as a osteoblast-specific factor. Periostin is a secreted protein that binds heparin and functions as a ligand for alpha(V)beta(3) and alpha(V)beta(5) integrins. In preosteoblasts, Periostin acts as a cell adhesion molecule and plays a role in osteoblast recruitment, spreading and attachment. Periostin is mainly detected in lower gastrointestinal tract, aorta, stomach, placenta, uterus and breast tissues but is up-regulated in epithelial ovarian tumors and overexpressed in breast cancer. Expression of Periostin is increased by bone morphogenetic protein (BMP2) and transforming growth factor β 1(TGF β 1). Periostin contains a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain, which shows homology with the insect protein fasciclin, and a C-terminal domain.骨膜素（PN），也称为成骨细胞特异性因子2（OSF-2），是最初被分离为成骨细胞特异性因子的二硫键连接蛋白。 骨膜素是一种分泌的蛋白质，与肝素结合，可作为alpha（V）beta（3）和alpha（V）beta（5）整联蛋白的配体。 在成骨细胞中，骨膜素充当细胞粘附分子，并在成骨细胞募集，扩散和附着中发挥作用。 骨膜素主要在下消化道，主动脉，胃，胎盘，子宫和乳腺组织中检测到，但在上皮性卵巢肿瘤中上调，在乳腺癌中过表达。 骨形态发生蛋白（BMP2）和转化生长因子β1（TGFβ1）增强了骨膜蛋白的表达。 骨膜素包含一个典型的信号序列，其后是一个富含半胱氨酸的结构域，一个四重重复结构域和一个C末端结构域，该结构域与昆虫蛋白fasciclin具有同源性。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-20437.htm
QQ:1749072012