ANTI-HUMAN CD45RA (HI100) BIOTIN
货号：13-0458-82
规格：100UG
价格：1620
产品类型：流式抗体
品牌：eBioscience
物种：人
宿主：小鼠
抗体亚型：IgG
荧光染料：Biotin
| 抗体类型: | 单抗 | 同型对照： | IgG2b, kappa |
| 免疫原性： | | 用法： | 0.5 µg/test(Flow） |
| Description: The HI100 monoclonal antibody reacts with human CD45RA, a 220 kDa molecule expressed by subpopulations of CD4+ peripheral T lymphocytes, CD8+ peripheral T lymphocytes, and B cells. The CD45RA+ T cell populations are mainly naive/virgin allowing the use of HI100 mAb as a phenotypic marker to discriminate T cell subsets.Applications Reported: The HI100 antibody has been reported for use in flow cytometric analysis.Applications Tested: The HI100 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2 µm post-manufacturing filtered. |
| 数据 |
| CD45RA Antibody (13-0458-82) in FlowStaining of normal human peripheral blood cells with Anti-Human CD45RA FITC. Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis. |
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| 参考文献： |
| 1.BloodFollicular lymphoma tumor-infiltrating T-helper (T(H)) cells have the same polyfunctional potential as normal nodal T(H) cells despite skewed differentiation."13-0458 was used in Flow cytometry/Cell sorting to evaluate the potential of FL-infiltrating CD4(+) T-helper cells to elicit antitumour effects."Authors Hilchey SP,Rosenberg AF,Hyrien O,Secor-Socha S,Cochran MR,Brady MT,Wang JC,Sanz I,Burack WR,Quataert SA,Bernstein SH |
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