Fas Ligand Rat ELISA Kit/大鼠Fas Ligand ELISA试剂盒
货号：ERFASLG,ERFASLGX5
规格：96 tests,5X96 tests
价格：7238,29826
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：大鼠
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 90 pg/mL |
| 检测范围 | 82.30-60,000 pg/mL |
样本类型/体积 | 上清液 100 μL 血清 50 μL 血浆 50 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | Fas Ligand (CD178) |
| Protein aliases | ADAM10-processed FasL form, APL, Apoptosis (APO-1) antigen ligand 1 (Fas antigen ligand), CD178, CD95 ligand, CD95-L, CD95L protein, Fas antigen ligand, Fas ligand, FasL ICD, FasL intracellular domain, Receptor-binding FasL ectodomain, sFasL, Soluble Fas ligand, SPA, SPPL2A-processed FasL form, Tumor necrosis factor (ligand) superfamily member 6 (apoptosis (APO-1) antigen ligand 1) (Fas antigen ligand), Tumor necrosis factor (ligand) superfamily, member 6 (apoptosis (APO-1) antigen ligand 1) (Fas antigen ligand), Tumor necrosis factor ligand superfamily member 6, membrane form, soluble form |
物种（Tested） | 大鼠 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | Apt1Lg1, CD95-L, Cd95l, Fasl, Faslg, Tnfsf6 |
| Gene ID | (Rat) 25385 |
| Gene symbol | Faslg |
| UniProt ID | (Rat) P36940 |
| The Rat Fas Ligand (FasL) ELISA quantitates Rt FasL in rat serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt FasL.大鼠Fas配体（FasL）ELISA定量了大鼠血清，血浆或细胞培养基中的大鼠FasL。 该检测试剂盒将专门识别天然和重组大鼠FasL。Principle of the method 方法原理The Rat FasL solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.Rat FasL固相夹心ELISA（酶联免疫吸附测定）设计用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息CD178 (Fas ligand, FasL) is a type-II-membrane protein, whose N-terminus is in the cytoplasm and its C-terminal region extends into the extracellular space. Its receptor, FasR, is a cell-surface-type-I-membrane protein and a member of the tumor necrosis factor (TNF) and nerve growth factor (NGF) receptor family. As a member of the TNF-cytokine family CD178 induces apoptosis when interacting with FasR. CD178 may exist as either membrane bound (45 kD) or soluble forms (26 kD). The soluble protein can be released from cells upon cleavage by metalloproteinases. Binding of CD178 to Fas leads to oligomerization of the receptor and triggers apoptotic cell death through the interaction of other proteins. CD178 is predominantly expressed in activated T-lymphocytes and natural killer (NK) cells also it is expressed in the tissues of immune-privilege sites such as testis and eye. CD178 expression is also reported in various tissues as thymus, liver, ovary, lung, heart and kidney. It is assumed that induction of apoptosis through CD178 is predominantly involved in anti-viral immune responses. CD178 is a cell surface molecule belonging to the tumor necrosis factor family, binds to its receptor Fas, thus inducing apoptosis. Various cells express FAS, where CD178 is expressed predominantly on activated T cells. FAS and CD178 are involved in the down-regulation of immune reactions as well as T cell-mediated cytotoxicity. CD178 concentration has also been shown to be associated with atherosclerosis and inflammatory disease, in patients with hypertension. The Fas/ CD178 system has been shown to play a role in a number of human diseases, for example AIDS, hepatitis or cancer.CD178（Fas配体，FasL）是一种II型膜蛋白，其N端位于细胞质中，其C端区域延伸到细胞外空间。它的受体FasR是一种细胞表面I型膜蛋白，是肿瘤坏死因子（TNF）和神经生长因子（NGF）受体家族的成员。作为TNF细胞因子家族的成员，CD178与FasR相互作用时可诱导细胞凋亡。 CD178可以以膜结合（45 kD）或可溶形式（26 kD）形式存在。可溶性蛋白可以在被金属蛋白酶切割后从细胞中释放出来。 CD178与Fas的结合导致受体的寡聚，并通过其他蛋白质的相互作用触发凋亡性细胞死亡。 CD178主要在活化的T淋巴细胞和自然杀伤（NK）细胞中表达，还可以在免疫特权部位的组织（例如睾丸和眼睛）中表达。在胸腺，肝，卵巢，肺，心脏和肾脏的各种组织中也有CD178表达的报道。假定通过CD178诱导细胞凋亡主要与抗病毒免疫反应有关。 CD178是属于肿瘤坏死因子家族的细胞表面分子，与其受体Fas结合，从而诱导细胞凋亡。各种细胞表达FAS，其中CD178主要在活化的T细胞上表达。 FAS和CD178参与免疫反应的下调以及T细胞介导的细胞毒性。在高血压患者中，CD178的浓度也被证明与动脉粥样硬化和炎性疾病有关。 Fas / CD178系统已显示出在许多人类疾病（例如艾滋病，肝炎或癌症）中的作用。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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