Aldosterone Competitive ELISA Kit/Aldosterone 竞争性ELISA试剂盒

货号：EIAALD,EIAALDX10

规格：96 tests,10X96 tests

价格：7238,52892

产品类型：ELISA检测

品牌：Thermo Fisher

物种：其它

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	4.97 pg/mL
检测范围	3.906-4000 pg/mL
样本类型/体积	粪便提取物 100 µL 血浆 100 µL 血清 100 µL 上清液 100 µL 尿液 25 µL
操作时间	1 hr 20 min
测试时间	16 hr 45 min
Homogenous (no wash)	No
批间变异系数（CV）	19.5%
批内变异系数（CV）	6.9%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	96 Tests
组份	- 预包被96孔板 - 标准品 - 测定稀释液 - HRP-共轭检测抗体 - 洗涤液 - 显色剂 - 终止液 - 胶板盖
储存温度	-20°C
Protein name	Aldosterone
Protein aliases	C21H28O5
物种（Tested）	Chemical
试剂盒类型	Competitive ELISA
标记（染料）	HRP
检测抗体偶联物	HRP

The Aldosterone Competitive ELISA quantitates aldosterone in extracted serum, plasma, urine, dried fecal extracts or cell culture medium.醛固酮竞争性ELISA在提取的血清，血浆，尿液，粪便提取物或细胞培养基中定量醛固酮。Principle of the method 方法原理The Aldosterone Competitive ELISA Kit research-use-only kit is designed to quantitatively measure aldosterone present in extracted serum and plasma, or in urine, extracted dried fecal samples, and tissue culture media samples. An aldosterone stock solution is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into the provided plate coated with a capture antibody. An aldosterone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to aldosterone to each well. After incubation, the plate is washed and substrate is added. The substrate reacts with the bound aldosterone-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.醛固酮竞争性ELISA试剂盒用于定量测量提取的血清和血浆或尿液，粪便提取物和组织培养基中的醛固酮。 提供了醛固酮储备液以生成测定的标准曲线，所有样品均应从标准曲线上读出。 将标准品或稀释的样品吸移到提供的带有捕获抗体的平板中。 将醛固酮-过氧化物酶结合物添加到孔中的标准品和样品中。 通过向每个孔添加针对醛固酮的多克隆抗体来引发结合反应。孵育后，洗涤板并加入底物。 底物与结合的醛固酮-过氧化物酶缀合物反应。 短暂孵育后，终止反应，并在能够在450 nm处测量的微量滴定板读数器中检测所产生颜色的强度。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Aldosterone is a hormone produced by the adrenal glands that helps regulate blood pressure through increasing both the release of potassium and the uptake of water and sodium by the kidneys. It is essential for sodium conservation in the kidney, salivary glands, sweat glands and colon. It plays a central role in the regulation of the plasma sodium (Na+), the extracellular potassium (K+) and arterial blood pressure. It does so mainly by acting on the mineralocorticoid receptors in the distal tubules and collecting ducts of the nephron. It influences the reabsorption of sodium and excretion of potassium (from and into the tubular fluids, respectively) of the kidney, thereby indirectly influencing water retention or loss, blood pressure and blood volume. When dysregulated, aldosterone is pathogenic and contributes to the development and progression of cardiovascular and renal disease. Aldosterone has exactly the opposite function of the atrial natriuretic hormone secreted by the heart.醛固酮是肾上腺产生的一种激素，通过增加钾的释放以及肾脏对水和钠的吸收，有助于调节血压。 它对于肾脏，唾液腺，汗腺和结肠的钠保持至关重要。 它在调节血浆钠（Na +），细胞外钾（K +）和动脉血压中起着核心作用。 它的作用主要是通过作用于远端小管中的盐皮质激素受体并收集肾单位的导管。 它会影响肾脏对钠的重吸收和钾的排泄（分别从肾小管液中和向肾小管液中排泄），从而间接影响保水或流失，血压和血容量。 当失调时，醛固酮是致病的，并有助于心血管和肾脏疾病的发展和发展。 醛固酮具有与心脏分泌的心钠素相反的功能。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。

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