Mouse anti-Human IgE Secondary Antibody/鼠抗人IgE二抗

货号：MA5-14727

规格：100 µg

价格：4018

产品类型：二抗

品牌：Thermo Fisher

抗原：Human Myeloma IgE

物种：人

宿主：小鼠

抗体亚型：IgG2b, kappa

荧光染料：其它

抗体类型:	二抗	同型对照：
浓度：	1 mg/mL	用法：	Assay Dependent（ELISA）；Assay Dependent (IP); Assay Dependent (RIA); 1:500-1:2000 (WB)

产品详细信息Product MA514727 is a smaller package size of MII0204PG (formerly sold as a Seradyn product).靶标信息Thermo Scientific Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据：

Human IgE Secondary Antibody (MA5-14727) in WBWestern blot analysis of human IgE was performed by loading 0.5 µg of purified human IgE, IgM, IgA and IgG per well and 10 µL of PageRuler Prestained Protein Ladder (Product # 26616) per well onto a Novex® 4-20% Tris-glycine polyacrylamide gel. Proteins were transferred to a PVDF membrane using the G2 Fast Blotter (Product # 62288), and blots were blocked with 5% milk in TBST for at least 1 hour at room temperature. Human IgE was detected at ~75 kD using a human IgE monoclonal antibody (Product # MA5-14727) at a dilution of 1:1000 in blocking buffer overnight at 4C on a rocking platform, followed by an HRP-conjugated goat anti-mouse light chain secondary antibody at a dilution of 1:40,000 for at least 30 minutes at room temperature (left blot). To control for protein loading, a duplicate blot was probed with a mixture of goat anti-human IgG, IgM, and IgA (Product # 31128) and goat anti-human IgE (Product # A18801) antibodies at concentrations of 1 µg/mL, followed by a mouse anti-goat IgG-HRP secondary antibody (Product # 31400) at a dilution of 1:8000 (right blot). Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080). Human IgE Secondary Antibody (MA5-14727) in ELISASandwich ELISA analysis of human IgE was performed by coating wells of a 96-well plate with100 µL per well of a goat anti-human IgG (H+L) antibody (Product # 31119) diluted to a concentration of 1 µg/mL in carbonate/bicarbonate buffer (Product # 28382), and incubating overnight at 4C. The plate was washed and blocked with StartingBlock T20 (TBS) Blocking Buffer (Product # 37543) for at least 30 minutes at room temperature, and then 100 µL per well of human IgE, diluted in 1X PBS at concentrations ranging from 1-1000 ng/mL (left panel), and normal human serum or serum from an asthma patient on anti-asthma prescription medications (control serum, right panel), diluted in 1X PBS at dilutions ranging from 1:5 to 1:5000, was added to the plate and incubated for at least 1 hour at room temperature. The plate was washed with 150 µL per well of 1X TBST, and 100 µL of a human IgE monoclonal antibody (Product # MA5-14727) was added to the wells at a concentration of 1 µg/mL, and incubated for 1 hour at room temperature. The plate was washed and incubated with 100 µL per well of an HRP-conjugated goat anti-mouse IgG Fc secondary antibody (Product # 31439) at a dilution of 1:8000 for at least 30 minutes at room temp. Detection was performed by adding 100 µL per well of 1-Step Ultra TMB substrate (Product # 34028) and incubating for a few minutes at room temp. The reactions were stopped with TMB Stop Solution (Product # N600). Absorbances were read on a spectrophotometer at 450-550 nm. Human IgE Secondary Antibody (MA5-14727) in ELISASandwich ELISA analysis of human IgE was performed by coating wells of a 96-well plate with100 µL per well of a goat anti-human IgG (H+L) antibody (Product # 31119) diluted to a concentration of 1 µg/mL in carbonate/bicarbonate buffer (Product # 28382), and incubating overnight at 4C. The plate was washed and blocked with StartingBlock T20 (TBS) Blocking Buffer (Product # 37543) for at least 30 minutes at room temperature, and then 100 µL per well of human IgE, IgM, IgA, or IgG, diluted in carbonate/bicarbonate buffer (Product # 28382) at concentrations ranging from 1-1000 ng/mL, was added to the plate and incubated for at least 1 hour at room temperature. The plate was washed with 150 µL per well of 1X TBST, and 100 µL per well of a human IgE monoclonal antibody (Product # MA5-14727) was added to the wells at a concentration of 1 µg/mL, and incubated for 1 hour at room temperature. The plate was washed, and incubated with 100 µL per well of an HRP-conjugated goat anti-mouse IgG Fc secondary antibody (Product # 31439) at a dilution of 1:8000 for at least 30 minutes at room temperature. Detection was performed by adding 100 µL per well of 1-Step Ultra TMB substrate (Product # 34028) and incubating for a few minutes at room temperature. The reactions were then stopped with TMB Stop Solution (Product # N600). Absorbances were read on a spectrophotometer at 450-550 nm.

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

技术参数

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-21291.htm