Cortisol Competitive Human ELISA Kit/人皮质醇竞争性ELISA试剂盒

货号：EIAHCOR,EIAHCORX10

规格：96 tests,10X96 tests

价格：7238,52892

产品类型：ELISA检测

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	17.3 pg/mL
检测范围	100-3200 pg/mL
样本类型/体积	粪便提取物 50 µL 血浆 1 µL 唾液 12.5 µL 血清 1 µL 上清液 50 µL 尿液 6.25 µL
操作时间	1 hr 20 min
测试时间	1 hr 30 min
Homogenous (no wash)	No
批间变异系数（CV）	8.1%
批内变异系数（CV）	8.8%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	96 Tests
组份	- 预包被96孔板 - 标准品 - 测定稀释液 - HRP-共轭检测抗体 - 洗涤液 - 显色剂 - 终止液 - 胶板盖
储存温度	-20°C
Protein name	Cortisol
Protein aliases	C21H30O5
物种（Tested）	人
试剂盒类型	Competitive ELISA
标记（染料）	HRP
检测抗体偶联物	HRP

The Human Cortisol ELISA quantitates Cortisol in human serum, plasma, urine, saliva, dried fecal extracts or or cell culture medium.人皮质醇ELISA可以定量人血清，血浆，尿液，唾液，干燥的粪便提取物或细胞培养基中的皮质醇。Principle of the method 方法原理The Cortisol Competitive ELISA research-use-only kit is designed to quantitatively measure cortisol present in dried fecal extracts, saliva, urine, serum, plasma, and tissue culture media samples. Total cortisol is measured in extracted samples and in serum and plasma, and free cortisol in saliva and urine. A cortisol standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture mouse antibodies. A cortisol-peroxidase conjugate is added to the wells. The binding reaction is initiated by the addition of a monoclonal antibody to cortisol. After a 1-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound cortisol-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader at 450 nm.皮质醇竞争性ELISA研究试剂盒用于定量干燥的粪便提取物，唾液，尿液，血清，血浆和组织培养基样品中的皮质醇。 测量提取样品，血清和血浆中的总皮质醇，以及唾液和尿液中的游离皮质醇。 提供皮质醇标准品以生成测定的标准曲线，并从生成的标准曲线中读取所有样品。 将标准品或稀释的样品吸移到涂有抗体的透明微量滴定板中，以捕获小鼠抗体。 将皮质醇-过氧化物酶缀合物添加至孔中。 通过向皮质醇添加单克隆抗体来引发结合反应。 温育1小时后，洗涤板并加入底物。 底物与结合的皮质醇-过氧化物酶缀合物反应。 短暂孵育后，停止反应，并在微量滴定板读数器中于450 nm处检测所产生颜色的强度。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Cortisol is the most potent glucocorticoid produced by the human adrenal. It is synthesized from cholesterol and its production is stimulated by pituitary adrenocorticotropic hormone (ACTH) which is regulated by corticotropin releasing factor (CRF). ACTH and CRF secretions are inhibited by high cortisol levels in a negative feedback loop. In plasma a majority of cortisol is bound with high affinity to corticosteroid binding globulin (CBG or transcotin). Cortisol acts through specific intracellular receptors and affects numerous physiologic systems including immune function, glucose counter regulation, vascular tone, and bone metabolism.皮质醇是人肾上腺产生的最有效的糖皮质激素。 它是由胆固醇合成的，其生成受到垂体促肾上腺皮质激素（ACTH）的刺激，该激素受促肾上腺皮质激素释放因子（CRF）的调节。 在负反馈回路中，高皮质醇水平会抑制ACTH和CRF分泌。 在血浆中，大多数皮质醇与皮质类固醇结合球蛋白（CBG或转铁蛋白）具有高亲和力。 皮质醇通过特定的细胞内受体发挥作用，并影响许多生理系统，包括免疫功能，葡萄糖调节，血管张力和骨代谢。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。

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