17-Hydroxyprogesterone Competitive ELISA Kit/17-羟孕酮竞争性ELISA试剂盒

货号：EIA17OHP,EIA17OHPX10

规格：96 tests,10X96 tests

价格：7238,52892

产品类型：ELISA检测

品牌：Thermo Fisher

物种：其它

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	20.3 pg/mL
检测范围	24.69-6000 pg/mL
样本类型/体积	血浆 50 µL 血清 50 µL 尿液 25 µL 上清液 50 µL 粪便提取物 50 µL
操作时间	1 hr 20 min
测试时间	1 hr 30 min
Homogenous (no wash)	No
批间变异系数（CV）	8%
批内变异系数（CV）	6.6%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	96 Tests
组份	- 预包被96孔板 - 标准品 - 测定稀释液 - HRP-共轭检测抗体 - 洗涤液 - 显色剂 - 终止液 - 胶板盖
储存温度	-20°C
Protein name	17-Hydroxyprogesterone
Protein aliases	C21H30O3, OHPG
物种（Tested）	Chemical
试剂盒类型	Competitive ELISA
标记（染料）	HRP
检测抗体偶联物	HRP

The 17-Hydroxyprogesterone Competitive ELISA quantitates 17-Hydroxyprogesterone in serum, plasma, urine, dried fecal extracts or cell culture medium.17-羟孕酮竞争性ELISA可以定量测定血清，血浆，尿液，干燥的粪便提取物或细胞培养基中的17-羟孕酮。Principle of the method 方法原理The 17-Hydroxyprogesterone Competitive ELISA research-use-only kit uses a specifically generated antibody to measure 17-Hydroxyprogesterone (17HO-P) and its metabolites in reconstituted buffer samples, tissue culture media samples, urine and fecal samples, or in extracted serum and plasma. This kit is not recommended for serum or plasma samples without extraction. A 17HO-P standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into the provided plate coated with a capture antibody. A 17HO-P-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to 17HO-P to each well. After a 1-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound 17HO-P-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.17-羟孕酮竞争性ELISA试剂盒使用特异生成的抗体来测定重构缓冲液样品，组织培养基样品，尿液和粪便样品中或提取的血清中的17-羟孕酮（17HO-P）及其代谢产物等。不建议将该试剂盒用于未提取的血清或血浆样品。提供了17HO-P标准品以生成测定的标准曲线，所有样品均应从标准曲线中读出。将标准品或稀释的样品吸移到提供的带有捕获抗体的平板中。将17HO-P-过氧化物酶偶联物添加到孔中的标准品和样品中。通过向每个孔中添加针对17HO-P的多克隆抗体来启动结合反应。温育1小时后，洗涤板并加入底物。底物与结合的17HO-P-过氧化物酶偶联物反应。短暂孵育后，终止反应，并在能够在450 nm处测量的微量滴定板读数器中检测所产生颜色的强度。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息17-Hydroxyprogesterone (4-pregen-17-ol-3, 20-dione, 17HO-P, OHPG) is a steroid hormone from the androgen group that is found in mammals, reptiles, birds, and other vertebrates. 17HO-P is identical across all species and so this kit should measure 17HO-P from all sources. In humans, it is primarily synthesized in the adrenal glands, the gonads, testes, and placenta.17-羟孕酮（4-pregen-17-ol-3、20-dione，17HO-P，OHPG）是雄激素类固醇激素，存在于哺乳动物，爬行动物，鸟类和其他脊椎动物中。 17HO-P在所有物种中都是相同的，因此该试剂盒应从所有来源测量17HO-P。 在人类中，它主要是在肾上腺，性腺，睾丸和胎盘中合成的。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。

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