IL1RL1/ST2 Human ELISA Kit/人IL1RL1/ST2 ELISA试剂盒
货号：EHIAST2,EHIAST2X5,EHIAST2X10
规格：96 tests,5X96 tests,10X96 tests
价格：7238,31508,0
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 30.5 pg/mL |
| 检测范围 | 31.25-2000 pg/mL |
样本类型/体积 | 上清液 100 μL 血清 12.5 μL 血浆 12.5 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | ST2 |
| Protein aliases | growth stimulation-expressed, homolog of mouse growth, interleukin 1 receptor-related protein, stimulation-expressed, homolog of mouse growth stimulation-expressed, interleukin 1 receptor-like 1, Interleukin-1 receptor-like 1, Protein ST2 |
物种（Tested） | 人 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | DER4, FIT-1, IL1RL1, IL33R, ST2, ST2L, ST2V, T1 |
| Gene ID | (Human) 9173 |
| Gene symbol | IL1RL1 |
| UniProt ID | (Human) Q01638 |
| The Human IL1RL1/ST2 ELISA quantitates Hu IL1RL1 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL1RL1.人IL1RL1 / ST2 ELISA可以定量人血清，血浆，缓冲液或细胞培养基中的人IL1RL1。 该检测试剂盒将只识别天然和重组的人IL1RL1。Principle of the method 方法原理The Human IL1RL1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人IL1RL1固相夹心ELISA（酶联免疫吸附测定）设计用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的目标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息ST2 is a member of the interleukin 1 receptor family. Studies of ST2 in mouse suggested that this receptor can be induced by proinflammatory stimuli, and may be involved in the function of helper T cells.ST2 belongs to the interleukin-1 receptor family with three Ig-like C2-type (immunoglobulin-like) domains and a TIR domain. ST2 is a receptor for interleukin-33 (IL-33) which upon stimulation recruits the signaling molecules MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Reports also suggest its possible role in helper T-cell function as well as in cell growth. Three isoforms of ST2 have been reported where isoform C is a membrane form and isoform B is a secreted form. ST2 may be induced by proinflammatory stimuli, and may also be found in serum of autoimmune disease. Different isoforms are restricted to different tissues while high expression of this protein is evident in kidney, lung, placenta, stomach, skeletal muscle, colon and small intestine.ST2是白介素1受体家族的成员。小鼠ST2的研究表明，该受体可被促炎性刺激诱导，并可能参与辅助性T细胞的功能。 ST2属于白介素-1受体家族，具有三个Ig样C2型（免疫球蛋白样）域和一个TIR域。 ST2是白介素33（IL-33）的受体，经刺激后募集信号分子MYD88，IRAK1，IRAK4和TRAF6，随后磷酸化MAPK3 / ERK1和/或MAPK1 / ERK2，MAPK14和MAPK8。报告还表明其可能在辅助T细胞功能以及细胞生长中的作用。已经报道了ST2的三种同工型，其中同工型C是膜形式，而同工型B是分泌形式。 ST2可以由促炎性刺激诱导，也可以在自身免疫性疾病的血清中发现。不同的同工型仅限于不同的组织，而该蛋白在肾脏，肺，胎盘，胃，骨骼肌，结肠和小肠中的高表达是明显的。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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