IL-1R4/IL-1RL1 Human ELISA Kit/人IL-1R4/IL-1RL1 ELISA试剂盒
货号：EHIL1RL1,EHIL1RL1X5,EHIL1RL1X10
规格：96 tests,5X96 tests,10X96 tests
价格：7238,29891,52892
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 2 pg/mL |
| 检测范围 | 1.65-1200 pg/mL |
样本类型/体积 | 上清液 100 μL 血清 50 μL 血浆 50 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | ST2 |
| Protein aliases | growth stimulation-expressed, homolog of mouse growth stimulation-expressed, interleukin 1 receptor-like 1, interleukin 1 receptor-related protein, Interleukin-1 receptor-like 1, Protein ST2 |
物种（Tested） | 人 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | DER4, FIT-1, IL1RL1, IL33R, ST2, ST2L, ST2V, T1 |
| Gene ID | (Human) 9173 |
| Gene symbol | IL1RL1 |
| UniProt ID | (Human) Q01638 |
| The Human Interleukin 1 Receptor 4 (Hu IL-1R4/IL-1RL1) ELISA quantitates Hu IL-1R4 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-1R4.人白介素1受体4（Hu IL-1R4 / IL-1RL1）ELISA定量了人血清，血浆或细胞培养基中的人IL-1R4。 该检测试剂盒将专门识别天然和重组的人IL-1R4。Principle of the method 方法原理The Human IL-11R4 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人IL-11R4固相夹心ELISA（酶联免疫吸附测定）设计用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息IL-33R, also known as ST2, is a receptor for IL-33. It is a member of the IL-1 receptor family of innate receptors. Its extracellular domain consists of 3 Ig-like domains that directly interact with its co-receptor IL-1RAcP, and the ligand alarmin, IL-33. IL-33 ligation results in the association of IL-33R complex with its adaptor proteins MyD88, IRAK1, IRAK4 and TRAF6, and activation of the NF-kB, and MAPK pathways. This, in turn, instigates the release of chemokines and cytokines such as IL-5, IL-6, IL-8, and IL-13. Two most common isoforms of IL-33R that result from alternative splicing are ST2L – the membrane bound form, and ST2S – the soluble form. The membrane bound form can be released from the cell surface by proteolytic cleavage. The soluble forms have been suggested to act as decoys dampening the IL-33 signaling. IL-33R is primarily expressed by mast cells, basophils, and eosinophils. In mouse, IL-33R expression has been shown on ILC2 and Th2 cells. IL-33R signaling has been implicated in the number of immune conditions, including allergies, arthritis, atherosclerosis and sepsis.IL-33R，也称为ST2，是IL-33的受体。它是先天性受体IL-1受体家族的成员。它的胞外域由3个Ig样域组成，它们直接与其共受体IL-1RAcP和配体警报蛋白IL-33相互作用。 IL-33的连接导致IL-33R复合物与其衔接蛋白MyD88，IRAK1，IRAK4和TRAF6缔合，并激活NF-kB和MAPK途径。反过来，这促使趋化因子和细胞因子如IL-5，IL-6，IL-8和IL-13释放。交替剪接产生的两种最常见的IL-33R亚型是ST2L（膜结合形式）和ST2S（可溶形式）。膜结合形式可以通过蛋白水解切割从细胞表面释放。已经提出可溶形式起抑制IL-33信号传导的诱饵作用。 IL-33R主要由肥大细胞，嗜碱性粒细胞和嗜酸性粒细胞表达。在小鼠中，IL-33R表达已显示在ILC2和Th2细胞上。 IL-33R信号传导与多种免疫状况有关，包括过敏，关节炎，动脉粥样硬化和败血症。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-20522.htm
QQ:1749072012