Cortisone Competitive ELISA Kit/Cortisone竞争性ELISA试剂盒

货号：EIACOR,EIACORX10

规格：96 tests,10X96 tests

价格：7238,52892

产品类型：ELISA检测

品牌：Thermo Fisher

物种：其它

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	29 pg/mL
检测范围	97.66-100,000 pg/mL
样本类型/体积	粪便提取物 50 µL 血浆 1 µL 唾液 10 µL 血清 1 µL 尿液 10 µL
操作时间	1 hr 20 min
测试时间	2 hr 30 min
Homogenous (no wash)	No
批间变异系数（CV）	11.9%
批内变异系数（CV）	8.6%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	96 Tests
组份	- 预包被96孔板 - 标准品 - 测定稀释液 - HRP-共轭检测抗体 - 洗涤液 - 显色剂 - 终止液 - 胶板盖
储存温度	-20°C
Protein name	Cortisone
Protein aliases	C21H28O5
物种（Tested）	Chemical
试剂盒类型	Competitive ELISA
标记（染料）	HRP
检测抗体偶联物	HRP

The Cortisone ELISA quantitates Cortisone in serum, saliva, urine, plasm, or dried fecal extracts.可的松ELISA可以定量测定血清，唾液，尿液，血浆或干燥的粪便提取物中的可的松。Principle of the method 方法原理The Cortisone Competitive ELISA research-use-only kit is designed to quantitatively measure cortisone in extracted dried fecal samples, urine, saliva, plasma, and serum samples. This kit measures total cortisone in serum and plasma and in extracted fecal samples. A cortisone standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. A cortisone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to cortisone to each well. After a two-hour incubation the plate is washed and TMB substrate is added. The substrate reacts with the bound cortisone-peroxidase conjugate to produce a colored product. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.可的松竞争性ELISA试剂盒用于定量测量提取的干燥粪便，尿液，唾液，血浆和血清样品中的可的松。该试剂盒可测量血清，血浆和粪便提取物中的总可的松。提供可的松标准品以生成测定的标准曲线，所有样品均应从标准曲线中读出。将标准品或稀释的样品吸移到涂有抗体的透明微量滴定板中，以捕获兔抗体。将可的松-过氧化物酶结合物添加到孔中的标准品和样品中。通过向每个孔中添加针对可的松的多克隆抗体来启动结合反应。温育两小时后，洗涤板并添加TMB底物。底物与结合的可的松-过氧化物酶缀合物反应以产生有色产物。短暂孵育后，终止反应，并在能够在450 nm处测量的微量滴定板读数器中检测所产生颜色的强度。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Cortisone (Kendall’s Compound E) is a glucocorticoid which is the product of cortisol (Compound F) deactivation by 11-beta-HSD2 (hydroxysteroid dehydrogenase), prohibiting receptor activation. The balance between cortisone and cortisol glucocorticoids helps to regulate the anti-inflammatory response. Cortisone is identical across all species.可的松（Kendall的化合物E）是一种糖皮质激素，是11-β-HSD2（羟基类固醇脱氢酶）使皮质醇（化合物F）失活的产物，阻止了受体的激活。 可的松和皮质醇糖皮质激素之间的平衡有助于调节抗炎反应。 可的松在所有物种中都是相同的。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。

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