BHQ-3 CPG; Glycolate, 500 Å
货号：BG5-5043G-1,BG5-5043G-100
规格：1 g,100mg
价格：23215.5,2940.6
产品类型：核酸合成
品牌：LGC Biosearch Technologies
| BHQ®-3 Glycolate CPG is used to add the non-fluorescent quencher BHQ-3 moiety to the 3' end of an oligonucleotide. BHQ-3 has maximal absorption in the 620 to 730 nm range, which provides excellent quenching of fluorophores emitting in this range. BHQ-3 Glycolate CPG has a glycolate linkage to the CPG support. The glycolate linker allows for rapid cleavage of the oligonucleotides and is labile enough for base sensitive oligonucleotides. The 500 Å CPG is useful for the synthesis of oligos up to 50-mers in length, especially when larger amounts of product are desired, as it allows for greater nucleoside loading amounts than do supports with larger pore sizes. |
| 特性： |
| - Appearance:dark blue powder- Absorption Maximum (Lambda Max):672- Extinction Coefficient at Lambda max:42700- Extinction Coefficient at 260 nm:13000 |
| 用途： |
Synthesis conditions: We recommend only using fast deprotecting amidites with this product due to the mild cleavage and deprotection conditions. Deprotection conditions: Cleave and deprotect in concentrated NH4OH for 1 hour at 60 °C (after 1 hour at 60 °C, degradation of product may occur). If product is left out in the open or exposed to heat for long periods degradation will occur. Image of cleaved and deprotected structure: The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:597.63
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