NFkB p65 (Phospho) [pS536] Human InstantOne ELISA Kit/人NFkB p65（磷酸）[pS536] InstantOne ELISA试剂盒
货号：85-86082-11
规格：96 tests
价格：6634
产品类型：InstantOne ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
样本类型/体积 | 细胞裂解液 20μL |
操作时间 | 1 hr 20 min |
测试时间 | 1 hr 10 min |
Homogenous (no wash) | No |
仪器设备 | Colorimetric microplate reader 酶标仪 |
规格 | 96 Tests |
组份 | - 捕获抗体 - 检测抗体 - InstantOne 检测板 - 细胞裂解液 - 增强剂溶液 - 洗涤液（10X） - 底物试剂（TMB） - 终止液 - 阳性对照细胞裂解物（冻干） - 密封条 |
储存温度 | 2-8℃ |
| Protein name | NFkB p65 |
物种（Tested） | 人 |
试剂盒类型 | InstantOne ELISA |
检测抗体偶联物 | Biotin |
标记（染料） | HRP |
| Gene ID | (Human) 5970 |
| Gene symbol | RELA |
Gene aliases | NFKB3, p65 |
UniProt ID | (Human) Q04206 |
| The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated human NFkB p65 in cell lysates. Detection of mouse and rat NFkB is expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.InstantOne™ELISA是专门设计用于准确测量细胞裂解物中磷酸化的人NFkB p65的。 预计将检测到小鼠和大鼠NFkB。 InstantOne™ELISA试剂盒可在大约一小时内快速分析样品。 将传统的夹心ELISA中使用的所有试剂均以溶液形式添加到板上，然后进行洗涤步骤，并使用TMB比色底物进行检测。Target information 靶标信息NF-kappaB (Nuclear Factor kappa B) is a nuclear transcription factor found in all cell types and is involved in cellular responses to stimuli such as stress, cytokines, free radicals, ultraviolet irradiation, and bacterial or viral antigens. NF-kappaB plays a key role in regulating the immune response to infection. Consistent with this role, incorrect regulation of NF-kappaB has been linked to cancer, inflammatory and autoimmune diseases, septic shock, viral infection and improper immune development. There are five members in the NF-kappaB family: NF-kappaB1, NF-kappaB2, RelA (also named p65), RelB, and c-Rel. The most common form of NF-kappaB is the p50/RelA heterodimer, although other forms of NF-kappaB dimers, such as p50/p50, p52/p52, p52/RelA, p50/c-Rel, c-Rel/c-Rel, p52/RelB, and p50/RelB, have also been identified in some types of cells. The primary role of NF-kappaB is to maintain normal cellular functions that range from cell-to-cell communication to cell motility, cell cycle progression, and cell lineage development. The activity of NF-kappaB is tightly regulated by interaction with inhibitory IkappaB proteins.NF-κB（核因子κB）是在所有细胞类型中发现的核转录因子，参与细胞对刺激的反应，例如应激，细胞因子，自由基，紫外线照射以及细菌或病毒抗原。 NF-κB在调节对感染的免疫反应中起关键作用。与此角色一致，NF-κB的不正确调节与癌症，炎性和自身免疫性疾病，败血症性休克，病毒感染和免疫发育不当有关。 NF-kappaB家族有五个成员：NF-kappaB1，NF-kappaB2，RelA（也称为p65），RelB和c-Rel。 NF-kappaB最常见的形式是p50 / RelA异二聚体，尽管其他形式的NF-kappaB二聚体，例如p50 / p50，p52 / p52，p52 / RelA，p50 / c-Rel，c-Rel / c- Rel，p52 / RelB和p50 / RelB也已在某些类型的细胞中鉴定出来。 NF-κB的主要作用是维持正常的细胞功能，其范围从细胞间通讯到细胞运动，细胞周期进程和细胞谱系发育。 NF-κB的活性受到与抑制性IkappaB蛋白相互作用的严格调控。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用，不用于诊断过程，未经明确授权不得转售。 |
| 数据： |
| NFkB p65 (Phospho) [pS536] Human InstantOne™ ELISA KitRepresentative data for NFkB p65 (Phospho) [pS536] Multispecies InstantOne™ ELISA. HeLa cells were unstimulated (-) or stimulated with 10 ng/mL TNF alpha for 5 min (+). Cells were analyzed by immunoblotting (left) and InstantOne™ ELISA (right). The intensity of the band at 65 kDa as detected by immunoblot correlates with InstantOne™ ELISA absorption values.NFkB p65（磷酸）[pS536]多物种InstantOne™ELISA的代表性数据。 未刺激（-）或用10 ng / mL TNFα刺激HeLa细胞5分钟（+）。 通过免疫印迹（左）和InstantOne™ELISA（右）分析细胞。 通过免疫印迹检测到的65 kDa的条带强度与InstantOne™ELISA吸收值相关。 |
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