CD279 (PD-1) Monoclonal Antibody (RMP1-30), FITC
货号：11-9981-81,11-9981-82
规格：50μg,100μg
价格：1132,1897
产品类型：流式抗体
品牌：Thermo Fisher
物种：小鼠
宿主：大鼠
抗体亚型：IgG2b, kappa
荧光染料：FITC
| 抗体类型 | 一抗 | 同型对照： | Rat IgG2b kappa Isotype Control (eB149/10H5), FITC |
| 浓度： | 0.5 mg/mL | 用法： | 0.5 μg/test (Flow)； |
| 产品详细信息Description: The RMP1-30 antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the Ig superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease in mice. PD-1 is expressed mainly on activated T and B lymphocytes. Two novel B7 Family members have been identified as PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these ligandsand their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. RMP1-30 does not block the binding of either B7-H1-Ig or B7-DC-Ig to PD-1 transfectants.Applications Reported: The RMP1-30 antibody has been reported for use in flow cytometric analysis.Applications Tested: The RMP1-30 antibody has been tested by flow cytometric analysis of Con A-stimulated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of theimmune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE. |
| 数据 |
| CD279 (PD-1) Antibody (11-9981-81) in FlowStaining of 3-day Anti-Mouse CD3 and Anti-Mouse CD28 Functional Grade Purified (Product # 16-0031-82andProduct # 16-0281-82)-stimulated C57Bl/6 splenocytes with 0.25 µg of Rat IgG2b K Isotype Control FITC (Product # 11-4031-82) (blue histogram) or 0.25 µg of Anti-Mouse CD279 (PD-1) FITC (purple histogram). Total viable cells, as determined by Fixable Viability Dye eFluor® 450 (Product # 65-0863-14), were used for analysis. |
| 推荐产品： |
| 二抗荧光二抗免疫组化一抗标签抗体 |
| 参考文献： |
| 1.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant SurgeonsNegative CD4 + TIM-3 signaling confers resistance against cold preservation damage in mouse liver transplantation."11-9981 was used in Flow cytometry/Cell sorting to explore TIM-3-Gal-9 function in a clinically relevant murine model of hepatic cold storage and orthotopic liver transplantation."AuthorsLiu Y,Ji H,Zhang Y,Shen XD,Gao F,Nguyen TT,Shang X,Lee N,Busuttil RW,Kupiec-Weglinski JW2.The Journal of experimental medicineCreatine uptake regulates CD8 T cell antitumor immunity."Published figure using CD279 (PD-1) monoclonal antibody (Product # 11-9981-82) in Flow Cytometry"3.Journal of immunology (Baltimore, Md. : 1950)Costimulator B7-DC attenuates strong Th2 responses induced by Nippostrongylus brasiliensis."11-9981 was used in Flow cytometry/Cell sorting to demonstrate that the inhibition of Th2 responses by B7-DC occurs independently of programmed death 1 but conceivably acts through an as yet unknown alternative receptor that enhances Th1 responses."4.Nature communicationsInterdependent IL-7 and IFN-γ signalling in T-cell controls tumour eradication by combined α-CTLA-4+α-PD-1 therapy."Published figure using CD279 (PD-1) monoclonal antibody (Product # 11-9981-82) in Flow Cytometry"5.The Journal of clinical investigationAntitumor adaptive immunity remains intact following inhibition of autophagy and antimalarial treatment."11-9981 was used in Flow cytometry/Cell sorting to evaluate how autophagy inhibition impacts the antitumor immune response in immune-competent mouse models of melanoma and mammary cancer." |
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-27780.htm
QQ:1749072012