TET Amidite, Single Isomer (N-TET-6-Aminohexanol)
货号：BNS-5033-100,BNS-5033-250,BNS-5033-50
规格：100 µmol,250 mg,50 µmol
价格：4361.7,10552.5,2251.2
产品类型：核酸合成
品牌：LGC Biosearch Technologies
| Tetrachlorofluorescein amidite is used for the 5' labeling of synthetic oligonucleotide probes used in 5' nuclease assays. TET has maximum absorbance at 523 nm and maximum emission at 540 nm. BHQ-1 dye will quench the TET moiety. |
| 特性： |
| - Chemical Name:[4,7,2',7'-tetrachloro-(3',6'-dipivaloylfluoresceinyl)-6-carboxamidohexyl]-1-O-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite- Formula:C46H54Cl4N3O10P- Molecular Weight:981.73- Appearance:white solid- Absorption Maximum (Lambda Max):523- Extinction Coefficient at 260 nm:16255- Fluorescence Maximum:540 |
| 用途： |
| Synthesis conditions:Prior to dilution ensure that all product is at the bottom of the vial. Dilute to the recommended concentration and mix thoroughly in the sealed vial to ensure that all contents are dissolved.Dilution:100 µmol/mLDeprotection conditions:With fast deprotecting amidites (ie. C-Ac, G-DMF, G-PAC) use concentrated NH4OH for 1 hour at 60°C. With standard amidites (ie. C-Bz, G-iBu) use concentrated NH4OH for 5 hours at 60°C. Image of cleaved and deprotected structure:The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:675.24 |
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