IgG3 Mouse Uncoated ELISA Kit with Plates/小鼠IgG3 ELISA试剂盒含未包被板
货号：88-50440-22,88-50440-86,88-50440-76
规格：2X96 Tests,10X96 Tests,20X 96Tests
价格：3125,6537,9393
产品类型：自定义ELISA
品牌：Thermo Fisher
物种：小鼠
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 1.875 ng/mL |
| 检测范围 | 1.875-125 ng/mL |
样本类型/体积 | 血浆 50 μL 血清 10 μL 上清液 10 μL |
操作时间 | 1 hr 45 min |
测试时间 | 24 hr 30 min |
Homogenous (no wash) | No |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 2 X 96 Tests |
组份 | - 捕获抗体：Pre-titrated, 纯化抗体 - 检测抗体：Pre-titrated, HRP偶联抗体 - 标准品：重组细胞因子，用于生成标准曲线和校准样品 - 10X包被缓冲液：用于包被捕获抗体的缓冲液 - 5XELISA/ELISPOT稀释液：封闭和稀释检测抗体和酶的缓冲液 - 底物：1X TMB溶液 - 分析证书：特定批次说明书用于抗体和标准品稀释 - 96孔板：Corning Costar 9018（含产品目录号，后缀 -22，-76，-86） |
储存温度 | 2-8℃ |
| Protein name | Mouse IgG3 |
物种（Tested） | 小鼠 |
试剂盒类型 | Uncoated ELISA Kit with Plates |
标记（染料） | HRP |
| 检测抗体偶联物 | HRP |
| The Mouse Immunoglobulin G3 (IgG3) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse IgG3. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.小鼠免疫球蛋白G3（IgG3）未包被的ELISA试剂盒包含预先匹配的抗体对，板和试剂，用于进行定量酶联免疫吸附测定（ELISA），以检测和定量小鼠IgG3的蛋白质水平。 需要洗涤缓冲液和终止液才能完成ELISA反应，需单独购买。Principle of the method 方法原理ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.ELISA被设计为测量匹配的抗体对之间结合的靶标的量。 将靶标特异性抗体包被到微孔板孔的底部，这是一整夜的过程。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Target information 靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.一抗的同种型及其应用可能导致背景染色。 一抗背景噪音可能是由于与靶细胞上的Fc受体结合而引起的。 通过与细胞蛋白质，碳水化合物和脂质的非特异性相互作用； 或通过细胞自发荧光。 同型对照抗体可以充当阴性对照，以帮助区分非特异性背景信号和特异性抗体信号，因为它们对靶抗原没有相关特异性。 尽管同型对照最常用于流式细胞术，但它们可用于其他应用，例如染色质免疫沉淀（ChIP），免疫组织化学和凝胶迁移。 同型对照应与一抗种类和同种型匹配，以便可以准确确定一抗特异性染色的水平。 如果使用直接标记的第一抗体，则同型对照如果与测试抗体标记相同，则效果最佳。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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