IgE Human Uncoated ELISA Kit/人IgE未包被ELISA试剂盒
货号：88-50610-77,88-50610-88
规格：20X96 Tests,10X96 Tests
价格：9393,6261
产品类型：自定义ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：IgE
荧光染料：其它
灵敏度 | 7.8 ng/mL |
| 检测范围 | 7.8-500 ng/mL |
样本类型/体积 | 血浆 10 μL 血清 10 μL |
操作时间 | 1 hr 45 min |
测试时间 | 24 hr 30 min |
Homogenous (no wash) | No |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 20 X 96 Tests |
组份 | - 捕获抗体：Pre-titrated, 纯化抗体 - 检测抗体：Pre-titrated, Biotin偶联抗体 - 标准品：重组细胞因子，用于生成标准曲线和校准样品 - 10X包被缓冲液：用于包被捕获抗体的缓冲液 - 5XELISA/ELISPOT稀释液：封闭和稀释检测抗体和酶的缓冲液 - 酶：Pre-titrated Avidin-HRP - 底物：1X TMB溶液 - 分析证书：特定批次说明书用于抗体和标准品稀释 - 96孔板：Corning Costar 9018（含产品目录号，后缀 -22，-76，-86） |
储存温度 | 2-8℃ |
| Protein name | Human IgE |
物种（Tested） | 人 |
试剂盒类型 | Uncoated ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| The Human Immunoglobulin E (Hu IgE) Uncoated ELISA Kit contains pre-matched antibody pairs and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IgE. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.人免疫球蛋白E（Hu IgE）未包被的ELISA试剂盒包含预先匹配的抗体对和用于执行定量酶联免疫吸附测定（ELISA）的试剂，以检测和定量人IgE的蛋白质水平。 需要洗涤缓冲液和终止液才能完成ELISA反应，需单独购买。Principle of the method 方法原理ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.ELISA被设计为测量匹配的抗体对之间结合的靶标的量。 将靶标特异性抗体包被到微孔板孔的底部，这是一整夜的过程。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Target information 靶标信息IgE is one of the five classes of immunoglobulins found in mammals. Monomers consist of two heavy (epsilon) chains with 4 Ig-like constant domains, and two light chains. IgE plays an essential role in type I hypersensitivity (allergic responses such as hay fever, asthma, hives, anaphylatic shock) by binding to Fc receptors on basophils and mast cells. Crosslinking of IgE bound to Fc receptors induces degranulation of mast cells and basophils that promote allergic manifestations. IgE is also functionally important for immunity against parasites.IgE是在哺乳动物中发现的五种免疫球蛋白之一。 单体由两条具有4个Ig恒定结构域的重（ε）链和两条轻链组成。 IgE通过与嗜碱性粒细胞和肥大细胞上的Fc受体结合，在I型超敏反应（过敏反应，如花粉症，哮喘，荨麻疹，过敏性休克）中起重要作用。 与Fc受体结合的IgE交联诱导肥大细胞和嗜碱性粒细胞脱颗粒，促进过敏性表现。 IgE在功能上对于抵抗寄生虫也很重要。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。 |
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