IL-23 Human Uncoated ELISA Kit with Plates/人IL-23 ELISA试剂盒含未包被板
货号：88-7237-22,88-7237-86
规格：2X96 Tests,10X96 Tests
价格：3403,6673
产品类型：自定义ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 15 pg/mL |
| 检测范围 | 15-2000 pg/mL |
样本类型/体积 | 血浆 100 μL 血清 100 μL 上清液 100 μL |
操作时间 | 1 hr 45 min |
测试时间 | 24 hr 30 min |
Homogenous (no wash) | No |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 2X 96 Tests |
组份 | - 捕获抗体：Pre-titrated, 纯化抗体 - 检测抗体：Pre-titrated, Biotin偶联抗体 - 标准品：重组细胞因子，用于生成标准曲线和校准样品 - 10X包被缓冲液：用于包被捕获抗体的缓冲液 - 5XELISA/ELISPOT稀释液：封闭和稀释检测抗体和酶的缓冲液 -样品稀释液A: 12 mL of a 1X solution per plate - 酶：Pre-titrated Avidin-HRP - 底物溶液：Tetramethylbenzidine (TMB) Substrate Solution - 分析证书：特定批次说明书用于抗体和标准品稀释 - 96孔板：Corning Costar 9018（含产品目录号，后缀 -22，-44，-76，-86） |
储存温度 | 2-8℃ |
| Protein name | IL-23 |
| Protein aliases | IL-23 subunit alpha, IL-23-A, Interleukin-23 subunit p19, IL-23p19, interleukin 23 p19 subunit, interleukin 23, alpha subunit p19, Interleukin-23 subunit alpha, interleukin-six, G-CSF related factor, JKA3 induced upon T-cell activation |
物种（Tested） | 人 |
试剂盒类型 | Uncoated ELISA Kitwith Plates |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | IL-23, IL-23A, IL23A, IL23P19, P19, SGRF, UNQ2498/PRO5798 |
| Gene ID | (Human) 51561 |
| Gene symbol | IL23A |
| UniProt ID | (Human) Q9NPF7 |
| The Human Interleukin 23 (IL-23) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-23. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.人白介素23（IL-23）未包被ELISA试剂盒包含预先匹配的抗体对，板和试剂，用于进行定量酶联免疫吸附测定（ELISA），以检测和定量人IL-23的蛋白质水平。 需要洗涤缓冲液和终止液才能完成ELISA反应，需单独购买。Principle of the method 方法原理ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.ELISA被设计为测量匹配的抗体对之间结合的靶标的量。 将靶标特异性抗体包被到微孔板孔的底部，这是一整夜的过程。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Target information 靶标信息IL-23 is a heterodimeric cytokine composed of the p40 subunit of IL-12 that is disulfide-linked with to two subunits, a p19 subunit unique to IL-23, and a p40 subunit that is shared with IL-12. IL-23 interacts with IL-12Rbeta1 and a beta2-like receptor subunit with STAT4 binding domain, IL-23R. IL-23 is secreted by activated mouse and human dendritic cells and is involved in the inflammation process and associated with autoimmune diseases. Mouse IL-23 was found not to induce significant amounts of IFN-gamma, induces strong proliferation of memory T cells (but not naive T cells), and activate mouse memory T cells to produce the proinflammatory cytokine IL-17. On the other hand, human IL-23 induces proliferation of memory T cells, induces moderate levels of IFN-gamma, and is produced by naive and memory T cells. IL-23 plays an important role in stimulating memory T-cells, and is required for the induction, expansion, maintenance, and downstream effector functions of Th17 cells, which play a vital role in upregulating neutrophil chemokines and various pro-inflammatory cytokines. IL-23 interacts with the receptors IL-12R beta1 and IL-23R to activate the Jak-Stat signaling cascade. IL-23 has been shown to possess potent anti-tumor and anti-metastatic activity in mouse models of cancer, suggesting a potential role for IL-23 in therapeutic treatment of cancer.IL-23是异源二聚体细胞因子，由与两个亚基二硫键连接的IL-12的p40亚基，IL-23独有的p19亚基和与IL-12共有的p40亚基组成。 IL-23与IL-12Rbeta1和具有STAT4结合结构域IL-23R的beta2样受体亚基相互作用。 IL-23由活化的小鼠和人树突状细胞分泌，并参与炎症过程并与自身免疫性疾病相关。发现小鼠IL-23不会诱导大量的IFN-γ，不会诱导记忆T细胞（而非幼稚T细胞）强烈增殖，并激活小鼠记忆T细胞以产生促炎性细胞因子IL-17。另一方面，人IL-23诱导记忆T细胞增殖，诱导中等水平的IFN-γ，并由幼稚和记忆T细胞产生。 IL-23在刺激记忆性T细胞中起重要作用，并且是Th17细胞的诱导，扩增，维持和下游效应子功能所必需的，Th17细胞在上调中性粒细胞趋化因子和各种促炎性细胞因子中起着至关重要的作用。 IL-23与受体IL-12R beta1和IL-23R相互作用以激活Jak-Stat信号级联反应。 IL-23已显示在癌症的小鼠模型中具有有效的抗肿瘤和抗转移活性，表明IL-23在癌症的治疗中具有潜在的作用。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。 |
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