IL-9 Mouse Uncoated ELISA Kit with Plates/小鼠IL-9 ELISA试剂盒含未包被板
货号：88-8092-76,88-8092-86,88-8092-22
规格：20X96 Tests,10X96 Tests,2X96 Tests
价格：9393,6537,3125
产品类型：自定义ELISA
品牌：Thermo Fisher
物种：小鼠
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 32 pg/mL |
| 检测范围 | 32-4,000 pg/mL |
样本类型/体积 | 血清 100 μL 上清液 100 μL |
操作时间 | 1 hr 45 min |
测试时间 | 24 hr 30 min |
Homogenous (no wash) | No |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 20 X 96 Tests |
组份 | - 捕获抗体：Pre-titrated, 纯化抗体 - 检测抗体：Pre-titrated, Biotin偶联抗体 - 标准品：重组细胞因子，用于生成标准曲线和校准样品 - 包被缓冲液：10X PBS ELISA包被缓冲液 - 检测稀释液：5X浓缩液 - 检测酶：Pre-titrated Streptavidin-HRP - 底物溶液：Tetramethylbenzidine (TMB) Solution - 分析证书：特定批次说明书用于抗体和标准品稀释 - 96孔板：Corning Costar flat-bottom plates（含产品目录号，后缀 -22，-76，-86） |
储存温度 | 2-8℃ |
| Protein name | IL-9 |
| Protein aliases | Cytokine P40, IL-9, T-cell growth factor P40, Interleukin-9 |
物种（Tested） | 小鼠 |
试剂盒类型 | Uncoated ELISA Kitwith Plates |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | Il-9, Il9, P40 |
| Gene ID | (Mouse) 16198 |
| Gene symbol | Il9 |
| UniProt ID | (Mouse) P15247 |
| The Mouse Interleukin 9 (IL-9) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse IL-9. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.小鼠白介素9（IL-9）未包被的ELISA试剂盒包含预先匹配的抗体对，板和试剂，用于进行定量酶联免疫吸附测定（ELISA），以检测和定量小鼠IL-9的蛋白质水平。 需要洗涤缓冲液和终止液才能完成ELISA反应，需单独购买。Principle of the method 方法原理ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.ELISA被设计为测量匹配的抗体对之间结合的靶标的量。 将靶标特异性抗体包被到微孔板孔的底部，这是一整夜的过程。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Target information 靶标信息IL-9 is a 14 kDa cytokine originally named P40 and identified by its proliferative effects on T cell populations. The receptor, which is a heterodimer of the gamma chain portion of the IL-2 receptor and the IL-9R chain, activates Jak/STAT signaling pathways upon binding its ligand. Since the discovery of IL-9, numerous other functions have been observed. It induces Th17 and Treg differentiation in CD4+ T cells, IgE production in B cells, and the differentiation and proliferation of mast cells. IL-9 expression was initially observed in Th2 cells, but has since been found in Th17, eosinophil, and mast cells. Th9 cells, a newly discovered subset of CD4+ T cells, are characterized by the secretion of large amounts of IL-9 and IL-10. Th9 development is induced by stimulation of undifferentiated CD4+ with IL-4 and TGF beta. Th2 cells can also be driven towards a Th9 phenotype in the presence of TGF beta.IL-9是一种14 kDa细胞因子，最初命名为P40，并通过其对T细胞群体的增殖作用进行鉴定。 该受体是IL-2受体的γ链部分和IL-9R链的异二聚体，结合其配体后会激活Jak / STAT信号传导途径。 自从发现IL-9以来，已经观察到许多其他功能。 它在CD4 + T细胞中诱导Th17和Treg分化，在B细胞中诱导IgE产生以及肥大细胞的分化和增殖。 最初在Th2细胞中观察到IL-9表达，但此后在Th17，嗜酸性粒细胞和肥大细胞中发现。 Th9细胞是CD4 + T细胞的新发现子集，其特征是分泌大量IL-9和IL-10。 通过用IL-4和TGFβ刺激未分化的CD4 +诱导Th9发育。 在存在TGFβ的情况下，Th2细胞也可以被驱动向Th9表型发展。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。 |
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