Amino Modifier TEG mdC Amidite (5'-DMT-mdC(TEG-Amino-TFA))
货号：BNS-5044-100,BNS-5044-50,BNS-5044-250
规格：100 µmol,50 µmol,250 mg
价格：2110.5,1195.95,4924.5
产品类型：核酸合成
品牌：LGC Biosearch Technologies
| Amino Modifier TEG mdC amidite incorporates an amino functionality within an oligonucleotide sequence or on the 5' terminus. The amine group is attached to a modified cytidine residue via triethyleneglycol linker, making it less likely for the label to interact with the double stranded duplex.The trifluoroacetyl (TFA) protecting group on the primary amine comes off during normal deprotection with concentrated ammonia. |
| 特性： |
| - Chemical Name: N-4-(13-(4,7,10-trioxatridecane)triflouroacetylamino)-5-methyl-5'-(4,4'dimethoxytrityl)-3'-O-(2-cyanoethyldiisopropylphosphoramidite)-
2'deoxycitidine- Formula: C52H70F3N6O11P- Molecular Weight: 1043.12- Appearance: white solid- Extinction Coefficient at 260 nm: 7050 |
| 用途： |
| Synthesis conditions:Prior to dilution ensure that all product is at the bottom of the vial. Dilute to the recommended concentration and mix thoroughly in the sealed vial to ensure that all contents are dissolved. Dilution: 100 mg/mLDeprotection conditions:To avoid a secondary alkylation acryonitrile reacton, which occurs during normal cleavage and deprotection of amine modified oligos, flush column for 15 minutes with 1 mL of 10% diethylamine (Aldrich #471216) in MeCN. Rinse column with MeCN before cleavage and deprotection of the amine labeled oligo.
If using standard amidites [i.e., A(Bz), G(iBu)] deprotect in concentrated NH4OH for 5 hours at 60 °C. If using fast deprotecting amidites [i.e., C(Ac), G(DMF), G(PAC)] deprotect in concentrated NH4OH for 1 hour at 60 °C. Note: under these conditions the TFA group will be removed.Amine Labeling:
Amino Modifier TEG mdC reacts in a manner identical to a normal phosphoramidite monomer. If labeling the 5' terminus, the DMT group may be left on after the synthesis for reverse-phase cartridge purification.
Oligo should be cleaved, deprotected, and purified before labeling. For reporter groups, protein labels, NHS, and enzymes see manufacturer's labeling protocol. Image of cleaved and deprotected structure: The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:506.49 |
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