IL-1 beta Rat Uncoated ELISA Kit with Plates/大鼠IL-1 beta ELISA试剂盒含未包被板
货号：88-6010-22
规格：2 x 96 tests
价格：3125
产品类型：自定义ELISA
品牌：Thermo Fisher
物种：大鼠
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 39 pg/mL |
| 检测范围 | 39-5,000 pg/mL |
样本类型/体积 | 上清液 100 μL |
操作时间 | 1 hr 45 min |
测试时间 | 24 hr 30 min |
Homogenous (no wash) | No |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 2X 96 Tests |
组份 | - 捕获抗体：Pre-titrated, 纯化抗体 - 检测抗体：Pre-titrated,biotin偶联抗体 - 标准品：重组细胞因子，用于生成标准曲线和校准样品 - 10X包被缓冲液：用于包被捕获抗体的缓冲液 - 5XELISA/ELISPOT稀释液：封闭和稀释检测抗体和酶的缓冲液 - 酶：Pre-titrated Avidin-HRP - 底物：1X TMB溶液 - 分析证书：特定批次说明书用于抗体和标准品稀释 - 96孔板：Corning Costar 9018（含产品目录号，后缀 -22，-76，-86） |
储存温度 | 2-8℃ |
| Protein name | IL-1 beta |
| Protein aliases | IL-1 beta, interleukin-1 beta |
物种（Tested） | 大鼠 |
试剂盒类型 | Uncoated ELISA Kitwith Plates |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene ID | (Rat) 24494 |
| Gene symbol | Il1b |
| The Rat Interleukin-1beta (IL-1b) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of rat IL-1b. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.大鼠白介素-1β（IL-1b）未包被的ELISA试剂盒包含预匹配的抗体对，板和试剂，用于进行定量酶联免疫吸附测定（ELISA），以检测和定量大鼠IL-1b的蛋白质水平。 需要洗涤液和终止液才能完成ELISA反应，需单独购买。Principle of the method 方法原理ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.ELISA是测量匹配的抗体对之间结合的靶标的量。 将靶标特异性抗体包被到微孔板孔的底部，这是一整夜的过程。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Target information 靶标信息Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.白介素-1 beta（IL-1 beta）是单核细胞，巨噬细胞和树突状细胞表达的促炎细胞因子。响应炎症刺激，IL-1β合成为31 kDa的无活性前体，并累积在细胞质中。将前IL-1β切割成活性的17 kDa蛋白需要激活炎性小体，炎性小体是对病原体，应激条件和其他危险信号有反应的多蛋白复合物。炎性体激活触发caspase-1前体加工成其活性形式，进而切割前IL-1 beta。 IL-1β缺乏经典ER /高尔基体途径的信号序列肽，并通过一种另类和不完全了解的机制与caspase-1一起分泌。尽管IL-1β最常以其活性形式分泌，但在某些生物学条件下可检测到未切割蛋白的分泌。 IL-1 beta通过两个受体IL-1RI和IL-1RII发出信号，这两个受体均与IL-1 alpha共享。 IL-1β活性可以由IL-1受体拮抗剂（IL-1RA）调节，IL-1RA是许多细胞类型产生的蛋白质，通过竞争性抑制作用阻断受体结合。 IL-1β通过触发靶细胞中其他促炎细胞因子的产生并启动对感染和损伤的急性期反应，在先天宿主防御中发挥重要作用。 IL-1β水平升高与许多慢性炎症疾病相关，IL-1β中和抗体具有潜在的治疗价值。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。 |
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