5'-DMT-dA(Bz) Synthesis Column; 1000 Å
货号:CG1-1000-1,CG1-1000-15,CG1-1000-2,CG1-1000-5
规格:1 µmol,1.5 µmol,200 nmol,50 nmol
价格:56.28,84.42,49.312,38.056
产品类型:核酸合成
品牌:LGC Biosearch Technologies
5'-DMT-dA(Bz) Synthesis Column; 1000 Å is for use on DNA synthesizers with luer column fittings (ABI 394, Expedite, Biosearch 8700, etc.). The column is packed with 5'-DMT-dA(Bz)-Suc-CPG, which is used for the placement of dA on the 3' end of oligonucleotides. The 1000 Å CPG is best suited for the synthesis of DNA sequences up to 100-mers in length. |
特性: |
- Appearance: White Powder- Extinction Coefficient at 260 nm: 15200 |
产品用途: |
Cleavage conditions: Use concentrated ammonia for 90 minutes at 25 °C when using standard amidites or 1:1 ammonia:methylamine (AMA) for 25 minutes at 25 °C when using fast deprotecting amidites.Deprotection conditions: When using fast deprotecting amidites (eg. C-Ac, G-DMF, G-PAC) use concentrated ammonia for 1 hour or AMA for 30 minutes at 60 °C. When using standard amidites (eg. C-Bz, G-iBu) use concentrated ammonia for 5 hours at 60 °C. Image of cleaved and deprotected structure:The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is: 313.2 |
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