DABCYL Synthesis Column, 3' Succinate Linkage (5'-DMT-mdC(TEG-DABCYL)-Suc-CPG); 500 Å
货号：CG5-5025S-1,CG5-5025S-2,CG5-5025S-5
规格：1 µmol,200 nmol,50 nmol
价格：562.8,225.12,168.84
产品类型：核酸合成
品牌：LGC Biosearch Technologies
| Biosearch Technologies’ 5'-DMT-mdC(TEG-DABCYL)-Suc CPG is based on a modified cytosine residue linked to the DABCYL chromophore via a triethyleneglycol spacer. The 3' end is conjugated to the CPG support via succinyl linkage. This synthesis column, packed with 5’-DMT-mdC(TEG-DABCYL)-Suc-CPG, is specifically suited for the preparation of molecular beacons or fluorescent energy transfer probes. DABCYL is used as a quencher for fluorophore groups such as fluorescein, TAMRA and JOE. |
| 特性： |
| ▪ Appearance:orange powder▪ Absorption Maximum (Lambda Max):472▪ Extinction Coefficient at Lambda max:32000▪ Extinction Coefficient at 260 nm:14333 |
| 产品用途： |
| Synthesis conditions:Follow the instrument manufacturer's protocol when using this product.Cleavage conditions:Use concentrated ammonia for 90 minutes at 25 °C.Deprotection conditions:If using fast deprotecting amidites (eg. C-Ac, G-DMF, G-PAC)) deprotect in concentrated NH4OH for 1 hour at 60 °C. For standard amidites (eg. C-Bz, G-iBu) deprotect in concentrated NH4OH for 5 hours at 60 °C. If using base sensitive fluorophores (ie. TAMRA or Rhodamine based dyes) we recommend deprotecting oligonucleotide in t-butylamine/H2O (1:3) for 12 hours at 60 °CThe mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:757.77 |
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