CD19 Monoclonal Antibody (eBio1D3 (1D3)), PE-eFluor 610
货号：61-0193-82
规格：100μg
价格：2738
产品类型：流式抗体
品牌：Thermo Fisher
物种：小鼠
宿主：大鼠
抗体亚型：IgG2a, kappa
荧光染料：PE-eFluor 610
| 抗体类型 | 单抗 | 同型对照： | Rat IgG2a kappa Isotype Control (eBR2a), PE-eFluor 610 |
| 浓度： | 0.2 mg/mL | 用法： | 0.25 μg/test (Flow)； |
| 产品详细信息Description: The eBio1D3 (1D3) monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, MHC class II, and CD19 form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells.Applications Reported: This eBio1D3 (1D3) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio1D3 (1D3) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD19 is a member of the immunoglobulin superfamily and has two Ig like domains. The CD19 molecule is expressed on 100% of the peripheral B cells as defined by expression of kappa or lambda light chains. CD19 appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen. The receptor for CD19 is an important functional regulator of normal and malignant B cell proliferation, and is expressed in all B cell precursor leukemias. Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. CD19 is a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. Besides being a signal-amplifying coreceptor for the B cell receptor (BCR), CD19 can also signal independently of BCR co-ligation and is a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity |
| 数据 |
| CD19 Antibody (61-0193-82) in FlowBALB/c mouse splenocytes were stained with CD3 Monoclonal Antibody, FITC (Product # 11-0031-82) and 0.125 µg of Rat IgG2a kappa Isotype Control, PE-eFluor 610 (Product # 61-4321-82) (left) or 0.125 µg of CD19 Monoclonal Antibody, PE-eFluor 610 (right). Total cells were used for analysis. |
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| 参考文献： |
| 1.Cell cycle (Georgetown, Tex.)PLEKHA7 defines an apical junctional complex with cytoskeletal associations and miRNA-mediated growth implications."61-0193 was used in Immunocytochemistry-immunoflourescence to further investigate the function and interacting partners of PLEKHA7 at the zonula adherens."AuthorsKourtidis A,Anastasiadis PZ2.Cell cycle (Georgetown, Tex.)PLEKHA7 defines an apical junctional complex with cytoskeletal associations and miRNA-mediated growth implications."61-0193 was used in Immunocytochemistry-immunoflourescence to further investigate the function and interacting partners of PLEKHA7 at the zonula adherens."AuthorsKourtidis A,Anastasiadis PZ3.Frontiers in cardiovascular medicineThe Role of Allograft Inflammatory Factor-1 in the Effects of Experimental Diabetes on B Cell Functions in the Heart."Published figure using CD19 monoclonal antibody (Product # 61-0193-82) in Flow Cytometry"4.Frontiers in immunologyPeritoneal Cells Mediate Immune Responses and Cross-Protection Against Influenza A Virus."Published figure using CD19 monoclonal antibody (Product # 61-0193-82) in Flow Cytometry"5.Frontiers in immunologyProlonged Deleterious Influences of Chemotherapeutic Agent CPT-11 on Resident Peritoneal Macrophages and B1 Cells."Published figure using CD19 monoclonal antibody (Product # 61-0193-82) in Flow Cytometry" |
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