CD40L (Soluble) Human Instant ELISA Kit/人CD40L (可溶) 一步法ELISA试剂盒
货号：BMS239INST
规格：128 tests
价格：0
产品类型：Instant ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度： | 0.062 ng/mL | 产品规格： | 128 tests |
检测范围： | 0.16-10.0 ng/mL | 标记染料： | HRP |
仪器： | 比色酶标仪 | 储存： | -20°C |
操作时间： | 20 min | 蛋白名称： | CD154 (CD40 Ligand) |
测试时间： | 2 hr 10 min | 特异性靶标位点： | Soluble |
一致性（未洗涤）： | No | 物种（经过测试）： | Human |
批间变异系数： | 14.2% | 检测试剂盒类型： | 即用型ELISA 试剂盒 |
批内变异系数： | 6.8% | 检测抗体偶联物： | HRP |
| 同名基因： | CD154, CD40L, HIGM1, IGM, IMD3,T-BAM, TNFSF5, TRAP, gp39, hCD40L | 基因名称： | CD40LG |
样品类型/体积： | 血清 15 µL 血浆 15 µL 上清液 15 µL | 组分： | - 预包被96孔板 - 生物素化检测抗体 - 链霉亲和素-辣根过氧化物酶 - 样品稀释液，冻干 - 标准曲线条 - 样品稀释液 - 洗涤浓缩液 - 底物溶液 - 终止液 - 裂解液10X - 胶板盖 |
| The Human Soluble CD40 Ligand(Hu sCD40L ELISA quantitates Hu sCD40L in human serum, plasma, cell culture supernatants, or other body fluids. The assay will exclusively recognize both natural and recombinant Hu sICD40L.人可溶性CD40配体（Hu sCD40L ELISA）可以定量检测人血清，血浆，细胞培养上清液或其他体液中的Hu sCD40L，该检测试剂盒将识别天然和重组Hu sICD40L。Principle of the method 方法原理The Human sCD40Lsolid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人sCD40L夹心ELISA（酶联免疫吸附测定）是测量匹配的抗体对之间结合的靶标量。 已在孔中预包被了靶标特异性抗体。 将样品或对照添加到这些孔中，并与固定的（捕获）抗体结合。 通过将第二（检测）抗体与捕获抗体不同表位上的靶标结合形成三明治。 与酶结合的抗体结合形成的三明治。 经过孵育和洗涤步骤以去除微孔板中未结合的物质后，添加底物溶液，该溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息CD154 is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD154 stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD154 has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. Further, CD154 also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2. A defect in CD154 gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. CD154 is a member of the tumor necrosis factor family, and is expressed primarily on activated CD4+ lymphocytes, but also on mast cells, basophils, eosinophils and human dendritic cells. CD154 is also found on some epithelial cells, carcinomas and lymphoid dendritic cells. CD154 also appears to be involved in the transduction of regulatory signals for cellular functions such as B cell proliferation and differentiation.CD154是在T细胞表面表达的膜糖蛋白和分化抗原。在存在细胞因子的情况下，CD154刺激B细胞增殖和所有免疫球蛋白同种型的分泌。已经显示CD154在外周血单核细胞中诱导细胞因子的产生和杀肿瘤活性。此外，CD154还共同刺激活化的T细胞的增殖，并伴随着IFN-γ，TNF-α和IL-2的产生。 CD154基因的缺陷导致无法进行免疫球蛋白类别转换，并与高IgM综合征相关。 CD154是肿瘤坏死因子家族的成员，主要在活化的CD4 +淋巴细胞上表达，但也在肥大细胞，嗜碱性粒细胞，嗜酸性粒细胞和人树突细胞上表达。 CD154还存在于一些上皮细胞，癌和淋巴样树突细胞中。 CD154也似乎参与了细胞功能（例如B细胞增殖和分化）调控信号的转导。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
| 特点： |
| -极其简便快捷：操作时间仅15 min- 使用率更高：单独的标品板条，可检测96个样品- 适用客户：高度追求实验效率的客户 |
| 数据： |
CD40L (Soluble) Human Instant ELISA™ KitRepresentative standard curve for Human soluble CD40 Ligand Instant ELISA |
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