E-selectin (Soluble) Monkey Instant ELISA Kit/猴选择素E(可溶) 一步法ELISA试剂盒

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E-selectin (Soluble) Monkey Instant ELISA Kit/猴选择素E(可溶) 一步法ELISA试剂盒

货号:BMS649INST

规格:128 tests

价格:0

产品类型:Instant ELISA

品牌:Thermo Fisher

物种:小鼠

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度:

0.6 U/mL

产品规格:

128 tests

检测范围:

1.88-60 U/mL

标记染料:

HRP

仪器:

比色酶标仪

储存:

-20°C

操作时间:

20 min

蛋白名称:

E-selectin

测试时间:

3 hr 10 min

特异性靶标位点:

Soluble

一致性(未洗涤):

No

物种(经过测试):

Baboon

批间变异系数:

15%

检测试剂盒类型:

即用型ELISA 试剂盒

批内变异系数:

5%

检测抗体偶联物:

HRP

样品类型/体积:

血清 20 µL

血浆 20µL

上清液 20µL

组分:

- 预包被96孔板

- 生物素化检测抗体

- 链霉亲和素-辣根过氧化物酶

- 样品稀释液,冻干

- 标准曲线条

- 样品稀释液

- 洗涤浓缩液

- 底物溶液

- 终止液

- 裂解液10X

- 胶板盖

The Monkey Soluble Endothelial Leukocyte Adhesion Molecule-1 (ELAM-1) (Mk E-selectin) ELISA quantitates Mk sE-Selectin in Monkey serum, plasma, cell culture supernatants, or other body fluids. The assay will exclusively recognize both natural and recombinant Mk sE-Selectin猴可溶性内皮细胞白细胞粘附分子1(ELAM-1)(Mk E-选择素)ELISA定量了猴血清,血浆,细胞培养上清液或其他体液中的Mk sE-选择素。 该检测试剂盒将专门识别天然和重组Mk sE-选择素。Principle of the method 方法原理TheMonkeysE-Selectin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.猴sE-Selectin夹心ELISA(酶联免疫吸附测定)是测量匹配的抗体对之间结合的靶标量。 已在孔中预包被了靶标特异性抗体。 将样品或对照添加到这些孔中,并与固定的(捕获)抗体结合。 通过将第二(检测)抗体与捕获抗体不同表位上的靶标结合形成三明治。 与酶结合的抗体结合形成的三明治。 经过孵育和洗涤步骤以去除微孔板中未结合的物质后,添加底物溶液,该溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试,其标准包括敏感性,特异性,精密度和批次间一致性。 有关验证的更多信息,请参见手册。Target information 靶标信息E-selectin (CD62E, ELAM-1, and LECAM-1) is a 115 kD type I membrane protein and a member of the selectin family. E-selectin is highly expressed on activated endothelial cells (IL-2, TNF- alpha, other cytokines can increase expression) and can also be expressed on endothelial cells in the skin, bone marrow and placenta. E-selectin is involved in tethering and leucocyte rolling on activated endothelium at inflammatory sites and may also play a role in tumor metastasis and angiogenesis. E-selectin binds to both Siayl Lewis X (CD15s) and PSGL-1 (CD162). The HCD62E antibody has been shown to recognize human E-selectin and to be useful for flow cytometry. In vitro experiments have shown that IL-1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NFkB dependent signaling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.E-选择素(CD62E,ELAM-1和LECAM-1)是一种115 kD的I型膜蛋白,是选择素家族的成员。 E-选择蛋白在活化的内皮细胞上高表达(IL-2,TNF-α,其他细胞因子可以增加表达),也可以在皮肤,骨髓和胎盘的内皮细胞上表达。 E-选择蛋白参与栓系和白细胞在炎性位点在活化的内皮上的滚动,也可能在肿瘤转移和血管生成中起作用。 E-选择蛋白与Siayl Lewis X(CD15s)和PSGL-1(CD162)都结合。 已显示HCD62E抗体可识别人E-选择蛋白,可用于流式细胞仪。 体外实验表明,IL-1,TNFα和细菌壁成分(如脂多糖)在依赖NFkB的信号级联反应中诱导E-选择蛋白的转录。 E-选择蛋白已在多种炎症情况下与血管内皮相关。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
特点:
-极其简便快捷:操作时间仅15 min- 使用率更高:单独的标品板条,可检测96个样品- 适用客户:高度追求实验效率的客户
数据:

E-selectin (Soluble) Monkey Instant ELISA™ KitRepresentative standard curve for monkey soluble E-Selectin Instant ELISA
技术参数
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