5'-DMT-dA(Bz) “Twist” Column; 1000 Å
货号：GG1-1000-10
规格：10 µmol
价格：323.61
产品类型：核酸合成
品牌：LGC Biosearch Technologies
| 5'-DMT-dA(Bz) "Twist" Column; 1000 Å is a 10 µmol column packed with 5'-DMT-dA(Bz)-Suc-CPG, which is used for the placement of a deoxyadenosine (dA) base on the 3' end of the oligonucleotide sequence. The 5'-DMT-dA(Bz)-Suc-CPG support is linked to the CPG support through a succinyl linkage which can be cleaved in concentrated NH4OH or Methyl amine/ NH4OH(AMA). The dA has a benzoyl protecting group which is removed during the deprotection step using concentrated NH4OH or AMA. This support is compatible with fast deprotecting amidites and chemistry. |
| 特性： |
| ▪ Appearance:white powder▪ Extinction Coefficient at 260 nm:15200 |
| 产品用途： |
| Synthesis conditions:Follow the instrument manufacturer's protocol, with noted changes, when using this product. When setting up the instrument for synthesis choose the 10µmol or 15µmol program for a successful synthesis using these columnsCleavage conditions:Use concentrated ammonia for 30 minutes at 65 °C or 1:1 ammonia:methylamine (AMA) for 25 minutes at 25 °C.Deprotection conditions:When using fast deprotecting amidites (eg. C-Ac, G-DMF) use concentrated ammonia for 1 hour or AMA for 30 minutes at 60 °C. When using standard amidites (eg. C-Bz, G-iBu) use concentrated ammonia for 5 hours at 60 °C. Image of cleaved and deprotected structure: The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is：233.24 |
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