GeneJET Plasmid Midiprep Kit/GeneJET质粒中提试剂盒
货号：K0481,K0482
规格：25 preps,100 preps
价格：1697,6091
产品类型：DNA提取
品牌：Thermo Fisher
Thermo Scientific GeneJET Plasmid Midiprep Kit, sufficient for 100 preps, is designed for large scale isolation of high quality plasmid DNA from recombinantE. colicultures. The kit utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 200 µg of high copy number plasmid DNA. The purified plasmid DNA can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing,in vitrotranscription, and transfection of robust cell lines. |
优点： |
| -Efficient—high yields up to 200 µg of high quality plasmid DNA from 50 mL of bacterial culture-High concentration of DNA purified—> 400 ng/ µL-Convenient—spin column format, centrifuge or vacuum protocols-Fast—procedure takes 60 minutes, considerably faster than using gravity flow columns |
数据： |
图1.High concentration of plasmid purified with GeneJET Midiprep KitConcentrations of eluted DNA obtained after the purification of high-copy plasmid DNA using different kits for midscale plasmid DNA purification at maximal recommended bacterial cell culture volumes.图2.High yields of plasmid purified with GeneJET Midiprep KitYields of high-copy plasmid DNA obtained using GeneJET Plasmid Midiprep Kit and other kits for midscale plasmid DNA purification at maximal recommended bacterial culture volumes. |
组成成分： |
▪Resuspension Solution ▪Lysis Solution ▪Neutralization Solution ▪Endotoxin Binding Reagent ▪Wash Solution I (concentrated) ▪Wash Solution II (concentrated) ▪RNase A Solution ▪Elution Buffer (10 mM Tris-HCl, pH 8.5) ▪GeneJET Midi Purification Columns pre-assembled
with collection tubes (15 mL) ▪Collection tubes (15 mL) |
相关产品： |
| ▪DNA 片段标准品▪DNA ladder▪DNA 聚合酶▪PCR▪DNA纯化▪RNA纯化 |
技术参数 产品应用 • Isolated plasmid DNA is suitable for all conventional molecular biology procedures, including:
• FastDigest restriction enzyme digestion
• Automated fluorescent sequencing
• PCR
• Cloning
• Transformation
• In vitro transcription
• Transfection of robust cell lines
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