Universal Support Super Column (DMT-Ribose-Linker); 500 Å
货号：SCG5-3400-1,SCG5-3400-2,SCG5-3400-5
规格：1 µmol,200 nmol,50 nmol
价格：49.312,31.624,28.14
产品类型：核酸合成
品牌：LGC Biosearch Technologies
| This super column is packed with Universal Support CPG (DMT-Ribose-Linker); 500 Å. Oligonucleotide synthesis using universal support CPG is performed using standard synthesis protocols for 1.0 µmol, 200 nmol or 50 nmol scale. The CPG support does not contribute to the base sequence; therefore, it may be necessary to include a nonsense base as the 3' terminal base for sequence entry systems. The 500 Å pore size is best suited for the synthesis of DNA sequences up to 50-mers in length. |
| 特性： |
| ▪ Chemical Name:5' CPG Succinyl 2'-O-Acetyl-3'-O-DMT Uridine & 5' CPG Succinyl 3'-O-Acetyl-2'-O-DMT Uridine▪ Appearance:White Powder |
| 产品用途： |
| Synthesis conditions:Follow the instrument manufacturer's protocol when using this product. Include a nonsense base as the 3' terminal base for sequence entry systems.Deprotection conditions:For cleavage use ammonia/LiCl (15 mg LiCl in 1 mL concentrated aqueous ammonia, ca 0.1N LiCl) or methylamine/ammonia/LiCl for 90 minutes at 25°C. Filter off CPG support and collect supernant.Purification and Analysis: If the trityl has previously been removed from the oligo, the lithium salt is removed by precipitation from cold ethanol/salt or using Sephadex G-25 filtration (NAP). Alternatively, the crude TRITYL-ON oligonucleotide can be desalted and purified using commercially available reverse phase oligo purification cartridges (MicroPure II, BTI MP-1602)
ABI 3900 users: Columns should be compatible with standard ABI protocols. However, when using 50 nmol Super Columns, the following changes are recommended:
1) Change the head pressure/purge pressure/chamber pressure to 3.5 psi.
2) An additional oxidation step
3) OPTIONAL (but may help): extension of the coupling time to 20 secThe mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:0 |
| 数据： |
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